NEW BD Cytometric Bead Array Flex Set System - …

1 NEW BD Cytometric Bead Array flex Set SystemThe easiestway to multiplex with beadsTable of ContentsIntroduction ..3BD CBA flex Sets - The Most Flexible Multiplex Bead Array System Available ..3 Customize Your BD CBA flex Set Assay in a Few Easy Steps ..5BD FACSA rray Bioanalyzer and the BD CBA flex Set Assay System ..6A Perfect Combination for Superior Throughput ..6 NEW FCAP Array Analysis Software ..7BD CBA flex Sets - Ideal for Multiplex Quantitation of Phospho-Proteins ..10BD CBA flex Set Assay Performance.

2 12 New Flexible System with Superior Quality and Reproducibility ..12 Product List ..172 For Research Use Only. Not for use in diagnostic or therapeutic applications are either tested in-house or reported in the literature. See Technical Data Sheets for does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Becton Dickinson and Company is strictly prohibited.

3 Macintosh and Mac are trademarks of Apple Computer, Inc., registered in the US and other and Windows are registered trademarks of Microsoft flow cytometers are class I (1) laser Array is a registered trademark of Softflow, , BD Logo and all other trademarks are the property of Becton, Dickinson and Company. 2004-2005 Becton, Dickinson and Company. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, stored in retrievalsystems, or translated into any language or computer language, in any form or by any means.

4 Electronic, mechanical, magnetic, optical, chemical, manual, orotherwise, without prior written permission from BD CBA flex Sets - The Most Flexible Multiplex Bead Array System AvailableBD Biosciences has always provided the most reliable and complete multiplex bead Array we ve taken the performance you trust and the ease-of-use you rely on and made it ultimatelyflexible. In addition to your need for a flexible assay System , we also recognize the value ofyour precious samples and the importance of data reproducibility, so we ve engineered the newBD Cytometric Bead Array (CBA) flex Set System to maximize both.

5 Visit more the BD CBA flex Set System You Get: Reagents that are the easiest and most efficient to multiplex A multiplex System that requires no assay formulation regardless of plex size Quantitative results from a single, small-volume sample Superior performance requiring less total time and less hands-on time than competitive bead-based immunoassays Results in less time than competitor assays Simple assay protocols yielding superior data Automated sample acquisition and increased throughput with the plate-based BD FACSA rray bioanalyzer A complete System backed by the service and reliability you expect from BD BiosciencesIntroduction3 Unless otherwise specified.

6 All products are for Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for applications are either tested in-house or reported in the literature. See Technical Data Sheets for cytometry is an analytical tool that allows for thediscrimination of different particles on the basis of size andcolor. The BD CBA employs a series of particles with discretefluorescence intensities to simultaneously detect multiple solubleanalytes from a single serum, plasma, or tissue culturesupernatant sample.

7 The BD CBA, combined with flow cytometry,creates a powerful multiple analyte (multiplex) assay System . TheBD CBA System uses the sensitivity of amplified fluorescencedetection by flow cytometry to measure soluble analytes with aparticle-based immunoassay. The combined advantages of thebroad dynamic range of fluorescence detection via flowcytometry, and the efficient capturing of analytes via suspendedparticles coated with distinct capture antibodies enable theBD CBA to use fewer sample dilutions to determine analyteconcentration in substantially less time (compared toconventional ELISA).

8 The specific capture beads are mixed with recombinant proteinstandards or test samples, then incubated with phycoerythrin(PE)- conjugated detection antibodies to form sandwichcomplexes. Following acquisition of sample data using the flowcytometer, the sample results are generated using the new FCAPA rray new BD CBA flex Setsystem provides an open andconfigurable menu of bead-based reagents designed tobe the easiest method ofcreating multiplex our proprietaryconjugation chemistry, pairoptimization strategies, anddirect PE detection reagents,you can be assured ofconsistent and superior assayperformance in complexbiological samples.

9 Each antibody pair we develop is evaluated for dynamic range,sensitivity, and parallel titration to native biological samples. We have also specifically formulated the assay diluent and washbuffers for each assay type to reduce potentially detrimentaleffects on assay performance when using serum and plasmasamples. By avoiding a streptavidin-biotin-PE detection methodemployed by other assays, our direct PE detection reagentsminimize the risk of increased background often caused byendogenous biotin in serum and lysate samples.

10 Thus, BD CBAFlex Sets provide a reliable and flexible method for quantitativedetection of multiple analytes in a single serum, plasma, tissueculture supernatant, or cell lysate sample. For additional resources including instrument setup templates,please visit (continued)4 Unless otherwise specified, all products are for Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for applications are either tested in-house or reported in the literature. See Technical Data Sheets for CBA flex Set Assay Protocol Schematic.