Transcription of 腸内細菌科菌群の検出試験法(増菌培養法) NIHSJ-15-ST4:2011
1 1 ( ) nihsj -15-ST4: 2011 (Enterobacteriaceae) (VRBG) ISO 21528-1:2004 25 g 225 ml 37 C 18 2 1 ml EE 10 ml 37 C 24 2 VRBG 37 C 24 2 1) (Buffered Peptone Water; BPW) 121 C 15 * g NaCl g Na2 HPO4 12H2O g KH2PO4 g 1,000 ml pH * 2) (Buffered brilliant green bile Glucose broth EE )
2 10 ml 30 25 C pH * g g Na2 HPO4 g KH2PO4 g g g 1,000 ml * 3) (Violet Red bile Glucose Agar VRBG ) 85 100 mm 15 ml 25 C pH 2 * ** 4) (Nutrient Agar) 121 C 15 25 C pH g * g NaCl g 9 g 18 g ** 1,000 ml * ** 5) ( ) ISO 21528-1.
3 2004 ( 11 mm 120 mm 3 cm) 121 C 15 OF * g g g NaCl g g 9 g 18 g ** 1,000 ml pH * ** * g g g g NaCl g g g 9 g 18 g ** 1,000 ml 3 6) N,N,N',N'- -p - g 100 ml 1) 170 C 180 C( ) 2) ( )
4 121 C( ) 3) 37 C 1 C 4) 44 C 47 C 5) 6) 85 mm 100 mm 7) ( 3 mm) / / 8) 1 ml 9) pH 25 C pH 10) ( ) ( ) 11) g ( ) 1) 25 g 2) 225 ml 1 2 37 C 18 2 3) 1 ml EE 10 ml 37 C 24 2 4) EE 1 VRBG 37 C 24 2 5) ( ) 37 C 24 2 6) 10 4 37 C 24 2 7)
5 /25 g /25 g 6 1) ISO 21528-1:2004, Microbiology of food and animal feeding stuffs Horizontal methods for the detection and enumeration of Enterobacteriaceae Part1: Detection and enumeration by MPN technique with pre-enrichment 5 ( ) 37 C 24 2 1 ml EE 10 mlVRBG ( )37 C 24 2 1 2 37 C 18 2 25 g BPW 225 ml37 C 24 2 ( ) 37 C 24 2 /25 g 6 2011 9
6 ISO/TC34/SC9 AOAC International Japan Section AOAC International Japan Section SUNATEC