NOVO MAC - spcoenzymes.com

1 novo MACM ulti-Purpose bioformulation with a new BioS Multi-Action Microbial ConsortiumIntroducing novo MAC with a new BioS microbialconsortium that demonstrates superior enzymeperformance for use in multiple applications. TheBioS consortium in novo MAC exhibits a broadrange of degradation capabilities needed for a multi-purpose product efficacious in maintenance of drainline and grease traps, improving septic and wastedegradation and cleaning and odor their natural environment, bacteria produce hundredsof enzymes in response to the organics present in theirenvironment. They produce extracellular enzymes thatbreak down proteins, starches, fats, oils, greases andtoilet tissue into smaller particles outside the bacterialcell.

2 The bacteria then transport the smaller particlesacross their cell membrane for use as an energy sourceand for building of new cellular components. Sincebacteria detect the organics present as potential foodand produce specific enzymes to breakdown theseorganics, it is a very efficient system. Many differentenzymes are required to completely breakdown MAC BioS consortium produces key extracellularenzymes including amylase, cellulase, lipase andprotease. Drain lines, grease traps, septic systemsand surfaces are nutrient rich systems for many bacteria can utilize these organics asfood sources, it is the bacteria with the most rapidproduction of these key enzymes that provide themost dramatic effects.

3 The new BioS microbialconsortium is the next generation technology inmicrobial blends with degradation capability superiorto the present market leading MSB MAC is designed to provide exceptionalperformance for the following applications: Drain lines and grease traps degrades andeliminates organics found in drain lines andgrease traps. Regular addition of novo MACmaintains a cleaner and odor-free system. Septic and waste treatment maintains effectiveactivity in septic systems by eliminating the needfor excessive pumping. Eliminates odors caused byincomplete digestion of malodorous volatile fattyacids. Bathroom cleaner and odor controller penetratesinto cracks, crevices and pores of surfaces whereorganics accumulate, actually removing the organicsto leave a visually cleaner surface.

4 Provides long-termodor control by removing the organics that causeodors and preventing the return of new BioS microbial blend offers: A stable consortium of safe Bacillus spores Production of multiple enzymes providinga wide range of degradation capabilities A synergistic blend that works in concert toprovide superior performance across multipleapplications Excretion of high levels of amylase, cellulase,lipase and protease enzymes Ability to work under aerobic and anaerobicconditions Single product simplicity for multi-applicationflexibilityCellulase: Cellulose is the most abundant biologicalcompound on earth and is present in stains, grimeand organic deposits. The BioS consortium producescellulase enzymes that break down this large chaininsoluble molecule into much smaller water soluble,biodegradable 3.

5 BioS and MSB consortiums are each spotted ontoseparate disks placed on a Petri plate containing a carboxy-methylcellulose medium. As cellulase is produced and excreted,the carboxy-methylcellulose is broken down. After the plate isstained with a congo red solution, the zone of clearing becomesvisible around the colony. The BioS consortium produces asignificantly higher level of cellulase when compared to theMSB BioS CMC Plate Stained with Congo Red SolutionZone of ClearingProtease: Proteins are long chains of amino acids thatare tightly coiled into a particular structure suitable forthe function of specific proteins. Proteases are enzymesthat hydrolyze peptide bonds in proteins to break downthe large structure into smaller groups of amino to the high degree of charged amino acids inproteins they are usually very sticky, and the proteasesproduced by the BioS microbial blend help to reducethis stickiness by breaking down the protein into morereadily biodegradable 4.

6 BioS and MSB consortiums are each spotted ontoseparate disks placed on a Petri plate containing a casein skimmilk medium. As protease is produced and excreted, a zoneof clearing becomes visible where the skim milk has beenbroken down. The BioS consortium produces a significantlyhigher level of protease when compared to the BioS Casein PlateZone of ClearingFigure 1. Respirometry was used to monitor the effectiveness ofsynthetic sludge degradation by comparing the new BioS microbialblend and the MSB microbial blend as measured by oxygenconsumption. The cumulative uptake is 74% higher for the BioS microbial blend, indicating that the BioS consortium has far betterdegradation 2.

7 Differential media on Petri plates visibly demonstrate breakdownof organic compounds by producing a zone of clearing (hydrolysis) aroundeach colony. A given quantity of microbial culture is spotted onto a disk thatis placed on a Petri plate containing a solid media (substrate) that allowsvisualization of an enzyme reaction. As the targeted enzyme is produced anddiffuses throughout the agar, a zone of clearing is created. The BioS microbialblend demonstrates superior cellulase, lipase and protease enzyme productionwhen compared to the MSB blend. Both blends produce high levels Profile - BioS vs MSB Spore BlendsSize of zones of clearing (mm)after 24 - 48 hours9876543210 Cellulase Amylase Protease LipaseBioS MSBO xygen UptakeBioS vs MSB Spore Blends0 2 4 66005004003002001000 Time, daysMSBC umulative Oxygen Uptake, mg/LBioS Product CharacteristicsBacteria Counts x 107 cfu/ml (200 Billion/gal)

8 Bacteria Type Blend of Bacillus SporesSalmonella Not detectedAppearance Creamy whiteFragrance Pleasantly perfumedShelf-life Two years; maximum loss of log at recommended storage conditionsPerformance CharacteristicsCharacterized Enzyme Production Lipase,Protease, Amylase,CellulaseBacterial Pathways Aerobic & Facultative AnaerobicpH Range - range 38 - 145 F (3 - 63 C)Storage and HandlingStore in closed containers at 50 - 100 F (10 - 40 C).

9 Wash hands thoroughly with warm, soapy water aftercontact. Avoid eye Packaging 4 x 1 gallon case 5 gallon pail 55 gallon drumProduct Code: 7002027 Safety of BioS ConsortiumNovo MAC contains a blend of safe Bacillusmicroorganisms. Toxicity studies done by an independentlaboratory revealed that the BioS consortium has noacute oral toxicity, no acute dermal toxicity, and no acuteinhalation toxicity at maximal test dose. Acute dermalirritation and acute eye irritation studies classify theBioS consortium as non-irritating. The BioS consortium also does not elicit a skin product not approved for use in : Starch is a sugar storage molecule composedof repeating units of glucose present in most foodsubstances.

10 Starch contributes to soiling by causingparticles to stick more readily to surfaces. The amylaseproduced by the BioS microbial blend breaks down thestarch into a more readily biodegradable form, reducingthe stickiness of particles and making them easier 6. BioS and MSB consortiums are each spotted ontoseparate disks placed on a Petri plate containing a starchmedium. As amylase is produced and excreted, the starch isbroken down. After the Petri plate is stained with an iodinesolution, the zone of clearing becomes visible. As demonstrated,both the BioS and MSB consortiums produce considerablelevels of amylase. Starch Plate Stained with Iodine Solution MSB BioS Zone of ClearingMSB BioS Lipase PlateFigure 5.