PAGE

1 Polyacrylamide gel lectrophoresis BIOSCIENCE &BIOTECHNOLOGY BIOSCIENCE &BIOTECHNOLOGY , ( ) ( )

2 R nm 3 9 bp bp Da Da FSETSTOP RUNPAUSE1000 MODEL 1000VC , '- Bis N,N,N'.

3 N'- - e - - - - - - 3 2 11 3

4 - - - - - 0 50 100 105104 20%15%10%.

5 Constant Current Constant Voltage - N.

6 N'- 2 G-250 R-250 - 10000 90000 200000 CS 2M TM u Tris (25mM) SDS ( ) (192mM)

7 500mL HCl pH 1mg 1g 300mL 100mL 600mL 300mL 100mL 600mL 30% N,N'- 100mL Tris-HCl Tris) SDS (HCl) pH 100mL Tris-HCl Tris SDS (HCl) pH 100mL 10% 100mg 1mL ATTO e-PAGEL ATTO EzStainAQur( ) ATTO EzApply( ) ATTO ATTO III ATTO ( -R) 1 20 L 20 100 L L ex.

8 L L 1 mg/mL SDS (1%) 2 ( DTT) (1%) Tris-HCl )1mL(50mM) 2mL(20% 10mL AE-6530 mm AE-1410 EzRun - - mA min AE-1440 EzStandard )

9 AE-1340 EzStain AQua 40 60 AE-7305 - PAGEL-High min DNA AE-1360 EzStain Silver ( )

10 ( ) Ez 2,000 ( ) A T T OEz \336,400 AE-7350 PAGE WSE-1020 PAGE -R PAGE ATTOEz AE-1340 EzStain AQua AE-1360 EzStainSilver