高成功率PCR酶 KOD FX

1 11-03 PCR KOD FX(Code ) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN 1 .. 1 2 PCR .. 3 3 .. 4 4 .. 5 5 .. 10 6 .. 12 7 .. 12 -20 C NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research. No right under any other patent claim (such as the patented 5 Nuclease Process claims in US Patents Nos. 5,210,015 and 5,487,972), no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.

2 This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. The use of betaine in DNA polymerase reactions is covered by intellectual property, including Patent No. 6,270,962 and related patents issued or pending in other countries (" "), exclusively licensed to EPICENTRE Technologies Corporation, 726 Post Road, Madison, WI 53713, USA ("EPICENTRE"). The right to use betaine in conjunction with KOD DNA Polymerase in PCR is sublicensed by EPICENTRE to TOYOBO Co., Ltd. solely for research purposes. The purchase of this product conveys to the buyer a limited, non-exclusive, non-transferable right under the to use the purchased product containing betaine and KOD DNA Polymerase in PCR solely for research purposes.

3 No rights are granted to resell, repackage, or further sublicense. No other license is granted to the buyer, whether expressly, by implication, by estoppel or otherwise. 1 PCR KOD DNA Polymerase Long PCR PCR Polymerase 3 5 Exonuclease 2 Hot start PCR KOD FX PCR 144,535 19 KOD FX Taq DNA Polymerase Long PCR 11 DNA 40kb DNA 24kb cDNA PCR PCR GC PCR 1 KFX-101(200 ) KFX-101B KFX-101C KOD FX ( U/ l) 200 l 1 2 PCR Buffer for KOD FX* 3 2mM dNTPs 1ml 2 200 5 200 10 * 2 PCR Buffer for KOD FX -20 C -20 C KOD FX DNA tPA 24kb tPA.

4 Tissue-type plasminogen activator 021-5879 4900 2 2 PCR PCR KOD FX 2x PCR buffer for KOD FX 25 l 1 2mM dNTPs 10 l each 10pmol / l Primer #1 l M 10pmol / l Primer #2 l M Template DNA 1 l Genomic DNA 200 ng / 50 l Plasmid DNA 50 ng / 50 l cDNA 200 ng RNA ) / 50 lKOD FX ( l) 1 l U / 50 l Autoclaved, distilled water up to 50 l KOD FX Vortex spin down PCR PCR Tm 73 C 10kb Step down PCR Predenature : 94 C, 2min. Predenature :94 C, 2min. Denature : 98 C, 10sec. Denature : 98 C,10sec. Extension : 68 C, 1min. /kb Annealing : (Tm-5) C, 30sec.

5 Extension : 68 C, 1min. /kb 25 40cycles 25 40cycles Step down Predenature : 94 C 2min. Denature : 98 C 10sec. Extension : 74 C 1min. /kb Denature : 98 C 10sec. Extension : 72 C 1min. /kb Denature : 98 C 10sec. Extension : 70 C 1min. /kb Denature : 98 C 10sec. Extension : 68 C 1min. /kb Extension : 68 C 7min. 15 25 cycles 5 cycles 5 cycles 5 cycles 3 (Extension) 3 25 30 10kb PCR 30 40 3 1 PCR 1 PCR total 50 l 2 3 dNTPs dNTPs Mixture(2mM) (Code No.:NTP-201) 4 GC 22~35mer(Tm >60 C) 5 25~35mer Tm 65 C 3 GC rich 6 DNA PCR 2 PCR 1 PCR PCR PCR TA KOD TA TArget Clone -Plus-(Code No.)

6 :TAK-201) PCR TA 021-5879 4900 4 2 PCR KOD FX(DNA Polymerase) 3 5 Exonuclease / DNA MagExtractor -PCR & Gel clean up-(Code No.:NPK-601) 4 1 GC rich GC rich KOD FX PCR GC rich M1 1kb DNA Ladder1 KOD FX2 KOD -Plus-3 KOD -Plus-+5% DMSO4 A 5 B 6 C 7 Taq DNA /HindIII digest TGF- (GC 70%)12345M2M16712345M2M16 IGF2R [NM_000876] (GC 90% mRNA)M1 1kb DNA Ladder1 KOD FX2 A 3: D 4: E GCrich 5: F GCrich 6: G GCrich M2 /HindIII digest DNA10ng / 50 cDNA HeLa Total RNA50ng / 50 M1 1kb DNA Ladder1 KOD FX2 KOD -Plus-3 KOD -Plus-+5% DMSO4 A 5 B 6 C 7 Taq DNA /HindIII digest TGF- (GC 70%)12345M2M16712345M2M16 IGF2R [NM_000876] (GC 90% mRNA)M1 1kb DNA Ladder1 KOD FX2 A 3: D 4: E GCrich 5: F GCrich 6.

7 G GCrich M2 /HindIII digest DNA10ng / 50 cDNA HeLa Total RNA50ng / 50 PCR KOD FX 123M2M1 2 l/ 50 M1 1kb DNA Ladder1 -globin -globin -globin / HindIII digest123M2M1 2 l/ 50 M1 1kb DNA Ladder1 -globin -globin -globin / HindIII digestM2M1 2 l/ 50 M1 1kb DNA Ladder1 -globin -globin -globin / HindIII digest 5 2 KOD FX DNA 100ng DNA Human -globin 50 l 15pmoles KOD FX 1U 2 step cycle 30cycle PCR PCR KOD FX 1234MM1234M1234M1234M1234M1234 MKOD FXTaq DNA B C KOD -Plus- DNA1 / 50 2 1 ng3 10 ng4 100 ng -globin 94 C3min.* 30 30 cyclesM:1kb DNA Ladder1234MM1234M1234M1234M1234M1234 MKOD FXTaq DNA B C KOD -Plus- DNA1 / 50 2 1 ng3 10 ng4 100 ng -globin 94 C3min.

8 * 30 30 cycles30 cyclesM:1kb DNA Ladder 3 KOD FX DNA 40kb DNA 24kb cDNA 021-5879 4900 6 DNA -globin PCR TArget Clone -Plus-(Code No.:TAK-201) TA 96 KOD FX PCR 144,535 19 Taq DNA Polymerase Long PCR 10 DNA10ng / 50 M1 1kb DNA Ladder1 5kb2 10kb3 20kb4 30kb5 40kbM2 / HindIII digest DNA50 200ng / 50 M1 1kb DNA Ladder1 -globin -globin -globin -globin /HindIII digest DNA total RNA100ng / 50 M1 1kb DNA Ladder1 Homo sapienspolymerase (DNA directed), epsilon [NM_006231] Homo sapiensinsulin-like growth factor 2 receptor [NM_000876] Homo sapiensdystrophin [NM_004006] /HindIII digest Template: DNA Template: DNA Template.

9 CDNA 12345M2M11234 5M2M1123M2M1 DNA10ng / 50 M1 1kb DNA Ladder1 5kb2 10kb3 20kb4 30kb5 40kbM2 / HindIII digest DNA50 200ng / 50 M1 1kb DNA Ladder1 -globin -globin -globin -globin /HindIII digest DNA total RNA100ng / 50 M1 1kb DNA Ladder1 Homo sapienspolymerase (DNA directed), epsilon [NM_006231] Homo sapiensinsulin-like growth factor 2 receptor [NM_000876] Homo sapiensdystrophin [NM_004006] /HindIII digest Template: DNA Template: DNA Template: cDNA 12345M2M11234 5M2M1123M2M1 4 PCR ( 100,000 020 40 60 80 100 120 140 160 Taq DNA PolymeraseA Long PCR Pfu DNA PolymeraseKOD FX KOD -Plus- 7 5 1 2 DNA Human Adult Skeletal Muscle total RNA 100ng / 50 Homo sapiensdystrophin [NM_004006] 94 C14min. 30 cycles 35 cycles 40 cycles MM / HindIII digest DNA Human Adult Skeletal Muscle total RNA 100ng / 50 Homo sapiensdystrophin [NM_004006] 94 C14min.)

10 30 cycles 35 cycles 40 cycles 30 cycles 35 cycles 40 cycles MM / HindIII digest DNA 200ng / 50 tPA 24kbPCR 94 cycles94 C7min. M5 cycles5 cycles5 cycles20 cycles M / HindIII digest DNA 200ng / 50 tPA 24kbPCR 94 cycles94 cycles30 cycles94 C7min. M5 cycles5 cycles5 cycles20 cycles94 cycles5 cycles5 cycles5 cycles5 cycles5 cycles20 cycles20 cycles M / HindIII digest 021-5879 4900 8 3 DNA -globin DNA1: 6ng / 50 l 2: 12ng3: 25ng4: 50ng5: 100ng6: 200ng7: 400ng8: 800ngPCR 94 cycles30 cycles 94 cycles5 cycles5 cycles20 cycles94 cycles5 cycles5 cycles20 cycles1 2 3 4 5 6 7 8 M 1 2 3 4 5 6 7 81 2 3 4 5 6 7 8 M 1 2 3 4 5 6 7 8M / HindIII digest tPA 24kbM:1kb DNA Ladder -globin DNA1: 6ng / 50 l 2: 12ng3: 25ng4: 50ng5: 100ng6: 200ng7: 400ng8.