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荧光定量PCR完全攻略 - shinegene.org.cn
4、定量pcr 根据反应体系是否设有参照物以及是否与标本在同一管中进行扩增，定量pcr可分为四种 方法： ①有限稀释法，即倍比稀释法：通过稀释阳性样品直至靶基因不能再扩增为止，来设置已知
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Trizol使用说明书
www.shinegene.org.cn12、测O.D值定量RNA浓度。注：此方法提取RNA A260/A280值在1.6-1.8之间；产率估计：组织标本： （ug RNA/mg组织）1-10ug，培养细胞（ug RNA/106 Cell）：5-15ug。注：组织或细胞量过少，可 酌情减少Trizol用量；组织或细胞用量过多，会引起DNA对RNA的污染；高蛋白、脂肪或多 …
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