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Pre-Analytical Variables in the Coagulation Lab: Why Does ...
Coagulation testing requires platelet poor plasma Defined as < 10,000/ul platelet count • Critical for frozen plasma aliquots High platelet count will neutralize heparin activity • Lower PTT or Anti-Xa result Mask presence of Lupus Anticoagulant (false negative) Centrifuge @ 1500g for 15 min @ room temp RPM ≠ g
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