Example: confidence
Protein Identification using Mass Spectrometry
trypsin) for Peptide Mass Fingerprinting. • Use proteolysis, CID and peptide fragmentation to identify sections of protein sequence. (Bottom up). • Fragmentation analysis of the intact protein ‘Protein Sequencing’ (Top down). Not normally used for protein ID’s, more for PTM analysis. • All these approaches rely on database searching and
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