-Proteostain- Protein Quantification Kit-Rapid (500 tests ...

1 -Proteostain- Protein Quantification Kit-Rapid (500 tests , 2500 tests ). Technical Manual Lowry Bicinchoninate (BCA ) Biuret Bradford . Coomassie Brilliant Blue G (CBB) ( ) Bradford Coomassie Brilliant Blue G ( max =595. nm) ( ) 1 30 . ready-to-use CBB . BSA 10 g/ml 2,000 g/ml . 1 g/ml 50 g/ml . a). O b). ecnabrosbA. NH +. Na -O S + SO3- 3. N N. 0. 500 600 700 800. Coomassie Brilliant Blue G (CBB) Wavelength/ nm Coomassie Brilliant Blue G . a) b) (BSA 500 g/ml). <500 tests > CBB solution 1. 00 ml 2. Standard BSA solution (4,000 g/ml) ml 1. <2500 tests > CBB solution 1,000 ml 1. Standard BSA solution (4,000 g/ml) ml 2. 0 5 CBB solution 0 5 12 6.

2 - 600 nm - 96 - 20 l 200 l - ml . - Table 2 .. -CBB solution . - CBB solution .. - . 1 . 1) Standard BSA solution 1/2 0 2,000 g/ml BSA ( ) . 2) 1) BSA 6 l n=3 .. 100 l 100 l 100 l 100 l 1. 4,000 g/ml BSA 100 l Absorbance at 595 nm ddH2O 100 l 1/2 1/4 1/8 1/16. 4000 g/ml Standard BSA solution 1/2 0. 0 500 1000 1500 2000. 2000, 1000, 500, 250, 125, 63, 32, 0 g/ml BSA . Concentration of BSA ( g/ml). BSA Fig. 4 Standard BSA solution . PQ01: -Proteostain- Protein Quantification Kit - Rapid Revised February 12, 2016. 3) CBB solution 300 l .. 4) 1 . 5) 570 600 nm . 6) (BSA : 0 g/ml) . 7) BSA BSA ( ) . 8) . 2 [ ]. 1) Standard BSA solution(4,000 g/ml) 30 l 300 l (400 g/ml) 250 l.

3 1 ml 100 g/ml BSA solution . 2) 100 g/ml BSA solution 1/2 0 50 g/ml BSA ( ) . 3) 2) BSA 150 l n=3.. 4) CBB solution 150 l .. 5) 1 . 6) 570 600 nm . 7) (BSA: 0 g/ml) . 8) BSA BSA ( ). 9) . 10) Table 1 .. 500 l 500 l 500 l 500 l Absorbance at 595 nm 100 g/ml BSA 500 l ddH2O 500 l 1/2 1/4 1/8 1/16 0. 100 g/ml Standard BSA solution 1/2 50, 25, 0 20 40 60 80 100. , , , , , 0 g/ml BSA Concentration of BSA ( g/ml). Standard BSA solution . BSA .. 3 [ ]. 1) Standard BSA solution 1/2 0 2,000 g/ml BSA ( ). 2) 1) BSA 100 l . 3) CBB solution ml . 4) 30 1 . 5) (1 cm 1 cm) 600 nm . 6) (BSA: 0 g/ml) . 7) BSA BSA ( ) . 8) . 150 l 150 l 150 l 150 l 2.

4 Absorbance at 600 nm 4,000 g/ml BSA 150 l 1. ddH2O 150 l 1/2 1/4 1/8 1/16. 0. 4,000 g/ml Standard BSA solution 1/2 0 500 1000 1500 2000. 2000, 1000, 500, 250, 125, 63, 32, 0 g/ml BSA Concentration of BSA ( g/ml). BSA Standard BSA solution . ( ). PQ01: -Proteostain- Protein Quantification Kit - Rapid .. Table 1 . Table 1 . Chemical Concentration Chemical Concentration Detergent Salt Brij 35 % Sodium chloride 2 mol/l Brij 56 % Potassium chloride 2 mol/l Brij 58 % Sodium acetate mol/l Triton X-100 % Sodium bicarbonate mol/l Triton X-114 % Buffer Tween 20 % Citrate pH mol/l Tween 80 % MES pH mol/l SDS % Tris pH mol/l CHAPS 4% PBS Undiluted CHAPSO 4% HEPES pH mol/l MEGA 10 4% CHES pH mol/l Octyl- -D-glucoside % Reducing agent Organic sovent Glucose 2 mol/l Ethanol 10 % Glutatione mol/l Isopropanol 10 % Ascorbic acid mol/l DMSO 10 % Dithiothreitol mol/l Chelating agent EDTA mol/l DTPA mol/l * BSA 5%.

5 BSA . Table 2 . Table 2 . Protein Protein vs. BSA a). BSA Chymotrypsinogen A Transferrin Human IgG a) . ( BSA ). 1) M. M. Bradford, Anal. Biochem., 1976, 72, 248.. Dojindo Molecular Technologies, Inc.. 30W Gude Dr, Suite 260, Rockville, Maryland, 20850 2025-5 861-2202. Tel: +1-301-987-2667, Fax: +1-301-987-2687 Tel:096-286-1515 Fax:096-286-1525 URL: ( ) Tel:03-3578-9651 Fax:03-3578-9650. PQ01: -Proteostain- Protein Quantification Kit - Rapi