自己組織化単分子膜(SAMs)をつくりたい

1 (SAMs) . / .. One shot type Hydroxy type . NTA-SAM Formation Reagent [N475] Hydroxy-EG3-undecanethiol [H354]. Biotin-SAM Formation Reagent [B564] Hydroxy-EG6-undecanethiol [H355]. Carboxylic acid-SAM Formation Reagent [C488] Hydroxy-EG3-hexadecanethiol [H395]. Amine Coupling Kit [A515] Hydroxy-EG6-hexadecanethiol [H396]. Amino type 16-Hydroxy-1-hexadecanethiol [H394]. Amino-EG6-undecanethiol, hydrochloride [A483] 11-Hydroxy-1-undecanethiol [H337].. Amino-EG6-hexadecanethiol, hydrochloride [A505] 8-Hydroxy-1-octanethiol [H338]. 16-Amino-1-hexadecanethiol, hydrochloride [A458] 6-Hydroxy-1-hexanethiol [H339]. 11-Amino-1-undecanethiol, hydrochloride [A423] Amido type 8-Amino-1-octanethiol, hydrochloride [A424] 10-Amido-1-decanethiol [A510]. 6-Amino-1-hexanethiol, hydrochloride [A425] 7-Amido-1-heptanethiol [A509]. Carboxy type 5-Amido-1-pentanethiol [A508].. Carboxy-EG6-undecanethiol [C445].

2 Carboxy-EG6-hexadecanethiol [C463] Sulfobetaine3-undecanethiol [S350]. 15-Carboxy-1-pentadecanethiol [C429] NTA type 10-Carboxy-1-decanethiol [C385] Dithiobis(C2-NTA) [D550]. 7-Carboxy-1-heptanethiol [C386]. 5-Carboxy-1-pentanethiol [C387]. 10-Carboxydecyl disul de [C404].. 7-Carboxyheptyl disul de [C405].. 5-Carboxypentyl disul de [C406]. Carboxy disulfide type 4,4'-Dithiodibutyric acid [D524].. Dithiobis(succinimidyl undecanoate) [D537]. Dithiobis(succinimidyl octanoate) [D538]. Dithiobis(succinimidyl hexanoate) [D539].. Ferrocenyl type 11-Ferrocenyl-1-undecanethiol [F246]. 8-Ferrocenyl-1-octanethiol [F247]. 6-Ferrocenyl-1-hexanethiol [F269]. I .. Ox (Self-Asssembled Monolayers; SAMs). X. 1-5) 4, 6, 7). X Re . 4, 8, 9) 10, 11) 12-14).. 15-17) 18-20). SAMs SPR QCM . 21-24). ( . 25, 26) 27, 28) 29). - ) . ( 1) . S S S S S S S S S S S S S S S S S S S S S S S S.. SAMs Au .. etc.

3 1 . Free Fax:0120-021557 Free Dial:0120-489548. ( request) Tel:096-286-1515 Fax:096-286-1525. 149 ( ) .. II SAMs / .. SAMs 1) 1 . (1) Piranha . 2) . (2) mmol/l .. SAM . 2.. 1) (-SH) .. 3. (3) . (-S-S-) Au-S 2). van der Waals - . (4) . 4.. 1 Bare SAM . Au-S .. Piranha 30 = 3:1 . Piranha 10 15 . SAM . 2 Au(111) Piranha .. in situ STM . nm Au ( nm) 3 UV- . Au 3 .. 30 ( 3 3) R30 SAMs . 30) i) ii) iii) .. Au(100) Au(200) Au(220) . iv) SPR QCM RAIRS . Au(311) SAM .. SAMs .. 3, 31, 32).. 4, 6-14).. Au(111) 2 SAM .. < >.. 33, 34).. mmol/l .. SAM . SAMs .. 2 (a) Au(111) C10SH SAM in situ STM . (b) Au(111) < >.. III SAMs .. SAMs .. SAMs SAMs . SAMs 33, 35).. < >. 25 SAM SAM . 35, 36).. 3 Dithiobis(succinimidyl undecanoate) .. 3 SAM 4 SAM . 37).. Free Fax:0120-021557 Free Dial:0120-489548. ( request) Tel:096-286-1515 Fax:096-286-1525. ( ) 150.. / IV . SAM (1) (-CONH2) . 44). (2) (3) (4).

4 SAMs . SAMs SAMs SAMs .. (1) . (5) SAMs . SAMs SAMs . 38-40). Au-S . SAMs .. 45). ( ) . 46-48). ( 4) . SAMs .. SAMs .. SAMs . 49).. V SAMs . SAMs . (STM/AFM) X (XPS) . (SPR) (QCM) . (CV) . 4 . CV .. (2) . SAMs . CV SAM . P-39-5 . (1) . SAMs . 11-Amino-1-undecanethiol, hydrochloride(Code: A423).. ( 11-AUT,HCl). SAMs . 8-Amino-1-octanethiol, hydrochloride(Code: A424).. ( 8-AOT,HCl). SAMs . 41-43) . 6-Amino-1-hexanethiol, hydrochloride(Code: A425).. ( 6-AHT,HCl). (3) . (2) SAMs .. 350 .. 3 (111) .. ( 1,000 ) 30 .. ( ) (111) ..SAMs . mmol / l ( .. ) 5 .. (4) .. (-EG6OH) (-OH) . Free Fax:0120-021557 Free Dial:0120-489548. ( request) Tel:096-286-1515 Fax:096-286-1525. 151 ( ) .. (3) CV VI SAMs / . 1) . SPR QCM . SAMs Ag/AgCl (KCl ) Pt Plate V .. V V 50 mV/sec .. mol/l KOH CV ( . DNA SAMs SAMs . 15 ) .. 2) CV . SAMs . 1) SAMs . WSC(EDC)/NHS .. 2) SAMs .. 5 .. SAMs . 5 . 2. nmol/cm.

5 SH . SAM . 3) NTA SAMs Ni .. 11-AUT, HCl His-Tag . 4) SAMs .. 5 A.. SAMs .. -1123mV .. 8-AOT, HCl VI-1 SAMs .. Current / mol/l . SAMs . WSC(EDC) [Code: W001] N -Hydroxy succineimide(NHS) . NHS . -970mV ( 6) . 6-AHT, HCl -886mV 6 SAMs .. Kyo 10-Carboxy-1-decanethiol (Code: C385) . Carboxy-EG6-undecanethiol (Code: C445) Carboxy-EG6- Potential / V undecanethiol Hydroxy-EG3-undecanethiol (Code: H354) . 5 9:1 SAM . SAM . 49).. 5 .. 1) Hydroxy-EG3-undecanethiol 1 mmol/l . SAM . Free Fax:0120-021557 Free Dial:0120-489548. ( request) Tel:096-286-1515 Fax:096-286-1525. ( ) 152.. 2. 2) / 40 mW/cm UV (2) . / . mmol/l SATP SATP DMF TEA ( ).. 3) 3 SAMs 1 9 . DPDS DPDS 1 mg mmol/l TEA ( ). A 1 mmol/l 10-Carboxy-1-decanethiol DMF 1 1 1 ml . B 1 mmol/l Carboxy-EG6-Undecanethiol 1 mmol/l DNA DNA C mmol/l Carboxy-EG6-Undecanethiol mmol/l TEA ( ) . mmol/l Hydroxy-EG3-undecanethiol % SDS / 2 SSPE ( ) 300 mmol/l NaCl 20 mmol/l.

6 4) mol/l WSC mmol/l NHS NaH2PO4 H2O 2 mmol/l EDTA 2Na mmol/l SDS . pH (20x SSPE Code: MB07) . 5) (3) . 6) 100 1,000 g/ml 2 1) DNA in situ SPR . 7) 2 mg/ml NH2-PEG4-OH 2 a) SPR SF10 . NHS (Schotto Glass technologies ) . 10 mmol/l , 150 mmol/l ( 5 nm) ( 45 nm) .. NaCl . b) a) mmol/l ODT / . VI-2 SAM DNA ODT SAMs . SPR c) ODT ( . SH SAMs DNA . UV ) ODT . d) c) mmol/l MUAM / 2. 50). ( 7) DNA . 500 m . SAMs DNA array SPR . 50, 51) MUAM SAMs . DNA .. e) d) SAMs 40 nl, mmol/l SATP . S. O. NH2. 1 2 . Au N. O O. f) e) 40 nl mol/l Hydroxylamine mol/.. O S CH3. O. O. O. N. O l DTT mol/l EDTA mol/l . O O SS. ( ) 20 . N.. S N S CH3. H O. Au D ep ro tec t SH SAMs . O. g) f) SAMs 40 nl DPDS 2 . S N SH. H. Au dithiopyridyl SAMs ( e), f), g) . M a l e im id o O. N S. S N SPDP d) SAMs . N R. a d d it i o n O SAMs . S N. O. S. O. N -R S N. O. SS. DTT. ) . H N. H. Au O Au SAMs . R' T h io l 40 nl.

7 SH. a d d i ti o n S N. O. SS. R' h) DNA . H. Au - . 7 MUAM SAMs DNA . SPR . i) 1 mol/l DNA h) . 10 . (1) . j) SPR . 11-Mercaptoundecylamine (MUAM)[ . 8 mmol/l 15 . 11-Amino-1-undecanethiol, hydrochloride(Code: A423)]. DNA DNA . N -Succinimidyl-S -acetylthiopropionate (SATP).. Sulfosuccinimidyl-4-(N -maleimidomethyl)cyclohexane-1- . carboxylate (SSMCC). Triethanolamine hydrochloride (TEA). SPR P . Dithiothreitol (DTT).. 2,2-Dipyridyl disul de (DPDS). CCD . E t h y l e n e d i a m i n e t e t r a a c e t i c a c i d d i s o d i u m s a l t . dihydrate(EDTA)[EDTA 2Na(Code: N001)]. Texas 1-Octadecanethiol (ODT). Instruments . Sodium dodecyl sulfate (SDS). PM-FTIRRAS( . Sodium chloride -1. 1,000 4 cm narrow-band HgCdTe ) . Sodium dihydrogenphosphate monohydrate DNA . Sodium hydroxide DNA . Ethanol SAMs DNA . DNA . Free Fax:0120-021557 Free Dial:0120-489548. ( request) Tel:096-286-1515 Fax:096-286-1525.

8 153 ( ) .. VI-3. 10) J. C. Love, D. B. Wolfe, , M. L. Chabinyc, K. , G. M. / . Whitesides, R. G. Nuzzo, J. Am. Chem. Soc. , 2003, 125 , 2597.. 11) A. Carvalho, M. Geissler, H. Schmid, B. Micel, E. Delamarche, Langmuir , SAMs 2002, 18 , 2406.. 12) Z. Li, Chang, R. S. Williams, Langmuir , 2003, 19 , 6744. 35,36) 13) D. Y. Petrovykh, H. Kimura-Suda, A. Opdahl, L. J. Richter, M. J. Tarlov, L. J.. Whitman, Langmuir , 2006, 22 , 2578.. 14) S. Lee, J. Park, R. Ragan, S. Kim, Z. Lee, D. K. Lim, D. A. A. Ohlberg, R. S. Williams, J. Am. Chem. Soc. , 2006, 128 , 5745.. 15) W. Muller, H. Ringsdorf, E. Rump, G. Wildburg, X. Zhang, L. Angermaier, . (1) . Dithiobis(succinimidyl undecanoate)(Code: D537). W. Knoll, M. Liley and J. Spinke, Science , 1993, 262 , 1706.. 16) M. Mrksich, G. B. Sigal, G. M. Whitesides, Langmuir , 1995, 11 , 4383. ( DSU) 17) J. M. Brockman, A. G. Frutos, R. M. Corn, J.

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