1 Mixing ChartSeparateQuest DiagnosticsBlood CollectionThe stopper color alone does not indicate tube type also refer to the tube gentle inversionsunless otherwise notedLIGHT BLUERED/BLACKREDGREENTANLAVENDERROYAL BLUEGRAY, and White LabelYELLOW, and White Label with Yellow Horizontal StripeGRAY, and Yellow LabelYELLOW PLASTIC, and Yellow LabelLIGHT BLUE (TUBE MUST BE FILLED COMPLETELY)RED/BLACK(DO NOT USE GEL TUBES FOR TOXICOLOGY OR DRUG TESTING)REDGREENor TANLAVENDER or TANROYAL BLUEGRAYTUBES WITH OTHER ADDITIVESYELLOW (DRAWN LAST) M sodium citrate ( ) 3-4 Inversions Clot activator and gel for serum separation5 Inversions Clot activator5 Inversions (plastic)None (glass) Sodium heparin Lithium heparin Sodium heparin (glass) K2 EDTA (plastic) Spray-dried K2 EDTA Sodium heparin Na2 EDTA None (serum tube) Potassium oxalate/sodium fluoride Sodium fluoride/Na2 EDTA Acid Citrate Dextrose(ACD).
2 Solutions A/B additives Trisodium citrate , citric acid and dextrose (in g/L) Boric acid, sodium formateShake vigorously PreservativeFor coagulation determinations. NOTE: Certain tests may require chilled specimens. Follow recommendedprocedures for Collection and transport. Inversions prevent Separator Tube (SST ) for serum determinations inchemistry and serology. Contains separator gel and should notbe used for toxicology or drug testing. Inversions ensure mixing of clotactivator with Blood . Blood clotting time 30 serum determinations in chemistry and serology, and fortoxicology and drug testing.
3 Glass serum tubes are recommendedfor Blood banking. Plastic tubes contain clot activator and arenotrecommended for Blood banking. Inversions ensure mixingofclot activator with Blood and clotting within 60 plasma determinations in chemistry. Tube inversionsprevent lead determinations. This tube is certified to contain less than g/mL (ppm) lead. Inversions prevent for whole Blood hematology determinations and immunohematology testing (ABO grouping, Rh typing, antibody screening). Inversionsprevent trace-element, toxicology and nutritional-chemistrydeterminations.
4 Special stopper formulation provides low levels of trace glucose determinations. Oxalate and EDTA anticoagulants will give plasma samples. Sodium fluoride is the antiglycolytic agent. Inversions ensure proper mixing of additive and tube with liquid ACD for use in Blood bank studies, HLA phenotyping, DNA, paternity testing, culture and sensitivity (C&S) urine testing. Minimum urine volume is 5 mL. For lower volumes, submit refrigerated urine in a sterile container urinalysis testing. Inversions ensure preservative is properly mixed.
5 Note the fill lines. Do not under fill (<2 mL) or overfill (>10 mL).CitrateGel, serumNo gel, serumHeparinEDTAEDTAS odium Flouride(Glucose)Citrate ACDFIRSTLASTS topper/Label ColorUrine SpecimensAdditive/Inversions at CollectionLaboratory UseBottle/StopperOrder of DrawAdditiveFor other Blood , Serum or Plasma separation instructions, see the Specimen Collection and Handling section of this DirectoryQuest Diagnostics Specimen Collection TubesWhen using a winged Blood Collection set forvenipuncture and a coagulation (citrate) tube is thefirst specimen to be drawn, a discard tube should be drawn first.
6 The discard tube must be used to fill the Blood Collection set tubing s dead space with Blood but the discard tube does not need to be completely filled. This important step will ensure maintenance of the proper Blood -to-additive ratio of the Blood specimen. The discard tube should be a nonadditive or coagulation tube. For serum (no gel) tubes BOTTLESSee bottle labelCourtesy and Becton, Dickinson and CompanyNOTE: See inside back cover for Specimen Collection tubesTo detect microbial growth from Blood Digest brothBD Vacutainer Tube TypeStopper ColorNumber of InversionsEDTAL avender8-10 CitrateLight Blue3-4 SST with gelRed/Black5 SerumRed5 Sodium FluorideGray8-10 HeparinGreen8-10