RambaQUICK Salmonella Method - CHROMagar

1 Food and Water Industry RambaQUICK TM. Salmonella Method +. Broth enrichment CHROM agarTM Salmonella Plus RambaQUICKTM Salmonella Culture media For Rapid detection of Salmonella species including S. Typhi, S. Paratyphi and Lactose positive RambaQUICKTM Salmonella Method For Rapid detection of Salmonella species including S. Typhi, S. Paratyphi and Lactose positive in food samples +. Background From the CDC report Trends in Foddborne Ilness, 1996-2010: This year's report (released in CDC's Morbidity and Mortality Weekly Report [MMWR]).

2 Summarizes 2010 data from FoodNet and provides the best measure of trends in foodborne disease from 1996 2010. It confirms that Salmonella infection has not declined in 15 years, . One of the report recommendations to reduce the number of Salmonella infections is Enhancing laboratory testing and disease reporting to more quickly identify outbreaks and their causes . CHROM agarTM Salmonella Plus Culture media RambaQUICK Salmonella Method : Rapid and Simple ! Broth enrichment The RambaQUICK Salmonella Method was designed with the aim of simplifying the test RambaQUICKTM Salmonella procedure while maintaining a high level of sensitivity within a conventional culture methodology.

3 1 TWO IN ONE ENRICHMENT STEP RambaQUICK Salmonella Method VS ISO standard Method ISO 6579:2002. The RambaQUICK selective broth is a combination of the best functions found 1 1. in each of both conventional enrichments, Pre-enrichment in buffer Peptone Pre-enrichment in buffer Peptone the RVS and the MKTTn broths. After the Water for 18h at 37 C. DAY 1 Water for 18h at 37 C.. revivification phase in BPW, the Salmonella replicates at the exponential growth phase 2. 2 3. in the optimised RambaQUICK Salmonella Transfer 0,1ml of the pre-enrichment Transfer 0,1ml Transfer 1ml broth, which offers not only a highly into 10ml of RambaQUICK into 10ml into 10ml nutrient environment but also inhibits the Salmonella broth and incubate of RVS broth of MKTT broth growth of competitive flora.

4 For 7h at 41,5 C and incubate for 24h and incubate for 24h 3 at 41,5 C at 37 C. DAY 2. 2 FAST Method Streak 10 l of RambaQUICK in a .. negative in 2 days. CHROM agar Salmonella Plus plate and incubate 18-24h at 37 C. positive in 3 days.. 3 SIMPLE Reading and Confirmation 4 5. DAY 3. 6 7. one selective enrichment. 1: oxydase test XLD Second one culture plate. 2: rapid urease test 3: serology Plate Plate XLD. Plate Second Plate 4 MEETS ISO 6579:2002 Incubation 24h at 37 C. REQUIREMENTS.. by detecting lactose positive Salmonella 8. in intense colour on CHROM agar DAY 4 Nutritive CHROM agar and RambaQUICK are trademarks of Dr A.

5 Rambach Salmonella Plus. 24h at 37 C. Plate Salmonella including , A and lactose positive Salmonella Mauve . DAY 5 Confirmation Method Description RambaQUICKTM Total .. g/L .. Enrichment Broth Salts .. Peptone .. +. Powder Version / Jan-17. Selective mix .. Powder Storage at 15/30 C - pH: + Shelf Life: 2 years UP TO 11 DAYS. Powder Base : Total .. g/L Supplements FOR POSITIVE SAMPLES. Agar .. 1st : Liquid form .. CHROM agarTM Peptone and Yeast extract .. Storage at 15/30 C Shelf Life: 2 years Salmonella Plus Salts .. In order to obtain a white opaque background: Culture Media Chromogenic mix.

6 2nd : White Opaque Supplement (Powder): Storage at 15/30 C - pH: Storage at 15/30 C Shelf Life: 5 years LF-EXT-036. Powder Shelf Life: 2 years Scientific Publications on this product: available on Please read carefully the instructions for use (IFU document) available on Order References Manufacturer: CHROM agar Please use these product references when contacting your local distributor: 4 place du 18 juin 1940 75006 Paris - France RambaQUICKTM Salmonella Enrichment broth powder in 1000 ml pack (100 tests of 10ml): SQ001 Email: CHROM agarTM Salmonella Plus culture media powder in 5000 ml pack (250 plates of 20ml): SA162 Website.

7 RambaQUICKTM Salmonella Method Find your nearest distributor on to prepare 5000 ml of media (250 tests): 3 packs of SQ001 + 1 pack of SA162 Industria de Alimentos y Agua RambaQUICK TM. Salmonella Method +. Caldo de enriquecimiento Medio de cultivo RambaQUICKTM Salmonella CHROM agarTM Salmonella Plus Para la detecci n r pida de especies de Salmonella incluyendo , y lactosa positiva RambaQUICKTM Salmonella Method Para la detecci n r pida de especies de Salmonella incluyendo S. Typhi, S. Paratyphi y lactosa positiva en muestras de comida +.

8 Antecedentes Del informe del CDC Trends in Foodborne Illnes , 1996-2010: El informe de este a o (publicado en el Informe Semanal del CDC Morbidity and Mortality [MMWR]) resume los datos del 2010 de FoodNet y pone en evidencia la tendencia de las enfermedades transmitidas por los alimentos entre 1996-2010, confirmando que la infecci n por Salmonella no ha disminuido en 15 a os, .. Una de las recomendaciones del informe para reducir el n mero de infecciones por Salmonella es Incrementar las pruebas de laboratorio y la notificaci n de enfermedades para identificar m s r pidamente los brotes y sus causas.

9 Medio de cultivo CHROM agarTM Salmonella Plus M todo RambaQUICK Salmonella : R pido y Simple ! El m todo RambaQUICK Salmonella fue dise ado con el objetivo de simplificar el Caldo de enriquecimiento RambaQUICKTM Salmonella procedimiento de los an lisis, manteniendo un alto nivel de sensibilidad dentro de la metodolog a convencional de cultivos. 1 ETAPA DE ENRIQUECIMIENTO DOS M todo RambaQUICK Salmonella VS M todo est ndar ISO 6579:2002. EN UNO. El caldo selectivo RambaQUICK es una 1 1. combinaci n de las mejores funciones Pre enriquecimiento en agua de Peptona Pre enriquecimiento en agua de Peptona que se encuentran en los enriquecimientos D A 1 tamponada durante 18h a 37 C.

10 Tamponada durante 18h a 37 C.. convencionales RVS y MKTTn. Despu s de la fase de revivificaci n en agua de peptona 2. 2 3. tamponada, la Salmonella se replica en la Transfiera 0,1ml del pre- Transfiera 0,1ml fase de crecimiento exponencial en el caldo enriquecimiento a 10ml de caldo Transfiera 0,1ml a 10ml de caldo optimizado RambaQUICK Salmonella , que RambaQUICK Salmonella e incube a 10ml de caldo RVS e incube no s lo proporciona un entorno altamente durante 7h a 41,5 C MKTT e incube durante 24h a nutriente sino que tambi n inhibe el 3.