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Red Blood Cell (RBC) Lysis Protocols
6. Centrifuge immediately at 500 x g for 5 minutes at room temperature. Decant the supernatant. 7. Resuspend cells in 2 mL of Flow Cytometry Staining Buffer or buffer of choice and centrifuge as in Step 6. Decant supernatant. 8. Resuspend cells in an appropriate volume of Flow Cytometry Staining Buffer or buffer of choice. 9.
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