Standard Methods for the Analysis of Oils, Fats and ...

1 Standard Methods forthe Analysis ofOils, Fats and Derivatives1st Supplement to the7th EditionInternational Union of Pure and Applied ChemistryCommission on Oils, Fats and DerivativesFORMER CHAIRMEN1930 1933:S. Fachini (Italy)1933 1936: F. Rivals (France)1936 1938: V. Vesely (Czechoslovakia)1938 1940: Kaufmann (Germany)1946 1949: Voerman (Netherlands)1949 195 1: Williams (United Kingdom)1951 1953: Snell (United States)1953 1957: J. Vizern (France)1957 1961: Sturm (Switzerland)1961 1964/65: G. Wolff (France)1964/65 1969: Boekenoogen(Netherlands)1969 1973: E. Heinerth (Fed. Rep. ofGermany)1973 1977: Vos (Netherlands)1977 1981: C. Paquot (France)1981 1983: D. Firestone (United States)1983 1985: M.

2 Naudet (France)1985 1987: O. Levin (Sweden)1987 1989: Mrs J. Beare-Rogers (Canada)COMPOSITION OF THE COMMISSION 1 JANUARY 1991 Titular membersChairman: Pocklington (UnitedKingdom)Vice-Chairman: A. Dieffenbacher(Switzerland)Secretary: Hendrikse (Netherlands)A. Karleskind (France)Mrs (Hungary)Mrs A. Visap (Finland)G. Zwerenz (Austria)National representativesArgentina: BrennerAustralia: SabineBulgaria: Mrs N. YanishlievaCzechoslovakia: J. PokornDenmark: HoImGermany: H. WesselsFinland: R. LinkoGreece: D. BoscouHungary: Miss M. Jer nekIndia: HA. BhakareObserversT. Asahara (Japan)D. Firestone (United States)0. Levin (Sweden)Associate membersMrs A. Bonekamp [Nasnerl (Germany)H. Br schweiler (Switzerland)P.]

3 Dysseler (Belgium)T. Hashimoto (Japan)Mrs G. H lmer (Denmark) Koelman (Netherlands)F. Mordret (France)G. Ostermann (Germany)M. Pike (United Kingdom)Israel: A. DolevJapan: S. WatanabeMalaysia: GohNetherlands: A. KrabNew Zealand: D. IllingworthSpain: M. Gassiot MatasTurkey: T. TuglularUnited States: WaltkingUSSR: RoubailoM. Naudet (France)R. Ohison (Sweden)K. Stig-Andersen (Denmark)JUPAC Secretariat: Bank Court Chambers, 2 3 Pound Way,Templars Square, Cowley, Oxford 0X4 3YF, Great BritainInternational Union of Pure and Applied ChemistryApplied Chemistry DivisionCommission on Oils, Fats and DerivativesStandardMethods forthe Analysis ofOils, Fats and Derivatives1St Supplement to the7th Revised and Enlarged EditionPreparedfor publication byA.

4 DIEFFENBACHERN estec Research CentreVers-chez-les-BlancCR1000 Lausanne POCKLINGTONL aboratory of the Government ChemistFood and Bioscience DivisionQueens RoadTeddington, Middlesex, United KingdomOXFORDBLACKWELL SCIENTIFIC PUBLICATIONSLONDON EDINBURGH BOSTONMELBOURNE PARIS BERLIN VIENNA International Union ofPure and Applied ChemistryPublished byBlackwellScientific PublicationsEditorial offices:OsneyMead, Oxford OX2 OEL25 JohnStreet, London WC1N 2BL23 Ainslie Place, Edinburgh EH3 6AJ3 Cambridge Center, CambridgeMassachusetts 02142, USA54 University Street, CarltonVictoria 3053, AustraliaOther Editorial Offices:Librairie Arnette SA2, rue Casimir-Delavigne75006 ParisFranceBlackwell Wissenschafts-VerlagMeinekestrasse 4D-1000 Berlin 15 GermanyBlackwell MZVF eldgasse 13A-1238 WienAustriaAll rights reserved.

5 No part of thispublication may be reproduced. storedin a retrieval system, or any form or by any , mechanical, or otherwise without theprior permission of the Book Services LtdP0 Box 87 Oxford 0X2 ODT(Orders:Tel:0865 791155 Fax: 0865 791927 Telex: 837515)AustraliaBlackwell Scientific Publications(Australia) Pty Ltd54 University StreetCarlton. Victoria 3053(Orders:Tel:03 347-0300)Distributed in the USAand North America byCRC Press, Corporate Blvd, NWBoca RatonFlorida 33431 British LibraryCataloguing in Publication DataDieffenbacher. Methods for the Analysis ofoils, fats and TitleII. Pocklington, published 19871stSupplement issued 1992 Setby Excel Typesetters. Hong KongPrinted in Great Britain at theAlden Press, OxfordContents of the 1 St SupplementPrefaceto the 1st SupplementList of contents of all Methods published in the 7th Edition and in this 1st Supplement[pages v_viii}*The Commission on Oils, Fats, and Derivatives (1985 1990) [pages xv and xvij*Warning (revised) [page xvii]*Quality assurance (analytical quality control) General principles [pages xviii_xixj**Precision data for the following Methods published in the 7th Edition.]]]

6 determination of acid value (acidity), of iodine value, and of peroxide valueCorrigenda/amendments to be made to Methods published in the 7th EditionNew analytical Methods developed and adopted by IUPAC Commission since thepublication of the 7th Edition:Section 1: Oleaginous Seeds and Fruits (2 Methods )Section 2:Oils and Fats (15 Methods )(alphabetical index to all Section 2 Methods now provided)Section 5: Lecithins (8 Methods )Section 6: Emulsffiers (2 Methods )*Pagesto replace those in the to the 1 St SupplementSincethe publication of the 7th edition of the Standard Methods , the Commission hasdeveloped and adopted more than 20 new Methods . Of the Methods now reproduced inthis 1st Supplement, 15 have already been published in Pure and Applied sections containing Methods for lecithins and emulsifiers are now included.

7 Of themethods for lecithins, four (phospholipids by thin-layer chromatography and phosphoruscontent, phospholipid profile by high performance liquid chromatography, acetone-insoluble matter, and toluene-insoluble matter) have been specially developed byIUPAC Commission , the remainder being general Methods for oils and fats(published in the 7th edition). The precision of four of the five general Methods includedhas been evaluated by collaborative study and shown to be satisfactory for the Analysis provision of a new binder with this 1st Supplement to the 7th edition will allowthe new material to be added to the original material, and retained in one binder. In somecases, it may not be possible to insert the new material at exactly the right point due to theoriginal pagination sequence.

8 Subscribers are therefore permitted to photocopy the originalpages where necessary so that new Methods can be inserted in the correct has been pointed out that certain corrections need to be made to the texts of somemethods published in the 7th edition. Accordingly, notes on the corrections requiredhave been included with this 1st Supplement. It is recognized that quite a number of im-provements could be made to many of the texts in the 7th edition and it is hoped thatwhen preparing a future 8th edition such improvements can be incorporated. The editorswelcome comments by users of the Methods regarding the improvement of the presenta-tion of any of the Methods and would appreciate notice of any textual errors that may befound.

9 The opportunity has also been taken to include in this 1st Supplement certainprecision data that were not available when the 7th edition was prepared for quality controlAn important development since the appearance of the 7th edition has been the publica-tion (1990) of the JUPAC Harmonized Protocol for the Presentation of the PerformanceCharacteristics of Standardized Analytical Methods . Unfortunately the time available forpreparing the Methods in the 1st Supplement for publication has not allowed thepresentation of the performance characteristics of the majority of these Methods in therecommended IUPAC protocol. However, it is to be hoped that a future 8th edition willsee all the Methods presented according to the recommended in this 1st Supplement are the general principles of analytical qualitycontrol which have been reproduced from Pure and Applied Chemistry (Vol.)

10 62, No. 1,pp. 153 154). It will be found that a limited number of new Methods in this 1stSupplement have associated with them specific applications of analytical quality control,presented according to the IUPAC indexTo meet the request from users of the Methods published in the 7th edition, analphabetical index has now been provided to facilitate the location of the Methods inSection 2 (Oils and Fats).June 1991 ContentsForeword,ixThe Commission on Oils, Fats and Derivatives of the IUPAC, xWarning, xviiQuality assurance (analytical quality control), xviiiSection 1: Oleaginous Seeds and of the sample for analysisDetermination of the Physical of the impuritiesliiiGrindingAnal vsis of the Seeds and of moisture and volatile matter contentI.