STANDARD OPERATING PROCEDURE TEMPLATE

1 Report #10-097, 6-2011 Page 1 of 5 STANDARD OPERATING PROCEDURE TEMPLATE #1 CONTACT INFORMATION: PROCEDURE Title Differential Scanning Calorimetry (DSC) User Protocol PROCEDURE Authors Amy Tsui & Jeffrey Tok Date of Creation/Revision 6/1/2011 Name of Responsible Person Jeffrey Tok Location of PROCEDURE Nanoscience Building, Room 008 Approval Signature #2 THIS STANDARD OPERATING PROCEDURE (SOP) IS FOR A: Specific laboratory PROCEDURE or experiment Differential Scanning Calorimetry (DSC) User Protocol Generic laboratory PROCEDURE that covers several chemicals Examples: distillation, chromatography, etc.

2 Generic use of specific chemical or class of chemicals with similar hazards Examples: organic azides, mineral acids, etc. #3 PROCESS OR EXPERIMENT DESCRIPTION This PROCEDURE covers the operation of differential scanning calorimetry (DSC). DSC is used to measure heat flow needed to increase the temperature of a sample by a desired amount. The heat flow to the sample is measured with respect to a reference. Frequency: one time daily weekly monthly other:_____ Duration per Expt: ~ 2-3 hours per sample Report #10-097, 6-2011 Page 2 of 5 #4 SAFETY LITERATURE REVIEW & HAZARD SUMMARY Stanford s Chemical Safety Tool Kit.

3 It can be accessed via: Stanford s EH&S Chemical Hygiene Plan. It can be accessed via: #5 STORAGE REQUIREMENTS n/a #6 STEP-BY-STEP OPERATING PROCEDURE Step-by-Step Description of Your Process or Experiment Potential Risks if Step is Not Done or Done Incorrectly (if any) Step 1: Don personal protective equipment. appropriate street clothing (long pants, close-toed shoes) gloves; indicate type:_____Nitrile or latex_____ safety goggles safety glasses face shield lab coats other:_____ Skin may be sensitive to samples. Step 2: Check the location/accessibility/certification of the safety equipment that serves your lab including fume hoods, safety showers, spill kits, fire extinguisher, etc.

4 Step 3: Fill in logbook Step 4: Open the valve on the nitrogen cylinder. Regulator is already set do not touch. Hazards of compressed gas (N2), please refer to: Step 5: Event switch should be left ON at electronics; if not, turn it on Step 6: Open software TA Instrument Explorer > Double click the DSC icon Report #10-097, 6-2011 Page 3 of 5 Step 7: Click Control > Event > ON. This will start the cooler. Flow rate should be 50 mL/min. Step 8: Wait for flange temperature to reach -70oC before running experiment (about 20min) Step 9: Setup PROCEDURE : a. Choose STANDARD /Custom or Modulated depending on your experiment b.

5 Fill In: i. Sample Name ii. Save To Location iii. Weight of Sample iv. Comments v. Operator vi. Pan Step 10: Creating New PROCEDURE : c. Click Editor > New i. Example PROCEDURE : 1. Data storage ON (begin collecting data) 2. Equilibrate [-40C] (brings temperature to -40C) 3. Isothermal [5min] (holds current temperature for 5min) 4. Ramp [5deg/min] up to [100C] (heats at rate of 5deg/min to 100C) Step 11: Add Sample (do not handle with your hands Use tweezers and wear gloves) a. Control > Lid > Open b. Place reference pan centered on the rear stage c. Place sample pan centered on the closer stage d.

6 Control > Lid > Close Use tweezers and wear gloves! Skin may be sensitive to samples. Step 12: Click Green Arrow Button ( play ) to start program. Step 13: Remove sample and reference pan (Control > Lid > Open, then Control > Lid > Close) Step 14: Repeat as needed for all samples Step 15: Run Cell/Cooler Conditioning PROCEDURE until flange reaches room temperature (flow rate should be 50 mL/min) Step 16: Stop PROCEDURE (Red square button) Step 17: Close software Report #10-097, 6-2011 Page 4 of 5 Step 18: Shut gas valve Step 19: Sign off in Log Book Step 20: Cleanup work area and lab equipment.

7 Step 21: Remove PPE and wash hands. #7 EMERGENCY procedures A. Health-Threatening Emergencies (ex: fire, explosion, health-threatening hazardous material spill or release, compressed gas leak, or valve failure) 1. Call 9-911 (or 286 in the School of Medicine). 2. Alert people in the vicinity and activate the local alarm systems. 3. Evacuate the area and go to your Emergency Assembly Point (EAP): 106 Center of Engineering Plaza 4. Remain nearby to advise emergency responders. 5. Once personal safety is established, call EH&S at 725-9999 (or 286 in the School of Medicine).

8 6. Provide local notifications: Jeffrey Tok, 650-575-9884 If personnel exposed or injured: 1. Remove the injured/exposed individual from the area, unless it is unsafe to do so because of the medical condition of the victim or the potential hazard to rescuers. 2. Call 9-911 (or 286 in the School of Medicine) if immediate medical attention is required. 3. Call 725-9999 (or 286 in the School of Medicine) to report the exposure to EH&S. 4. Administer first aid as appropriate. 5. Flush contamination from eyes/skin using the nearest emergency eyewash/shower for a minimum of 15 minutes.

9 Remove any contaminated clothing. 6. Bring to the hospital copies of MSDSs for all chemicals the victim was exposed to. B. Non-Health Threatening Emergencies For non-health threatening injuries and exposures Call the Occupational Health Center at 725-5308 for more information and to schedule an appointment. For hazardous material spills or releases which have impacted the environment (via the storm drain, soil, or air outside the building) or for a spill or release that cannot be cleaned up by local personnel: 1. Notify Stanford University responders by calling 725-9999 (or 286 in the School of Medicine).

10 These services are available 24 hours a day, 7 days a week. 2. Provide local notifications: Jeffrey Tok, 650-575-9884 Identify the area management staff that must be contacted and include their work and home numbers. This must include the principal investigator and may include the lab safety coordinator, facilities manager, and/or business manager. [PRECEDING GUIDANCE TEXT MAY BE DELETED] C. Small Spills/Local Cleanup: Report #10-097, 6-2011 Page 5 of 5 In the event of a minor spill or release that can be cleaned up by local personnel using readily available equipment (absorbent, available from EH&S in Small Spill Kit): 1.