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Final DNA Concentration in PicoGreen Assay 1000 0 1000 1000 ng/mL 500 500 1000 500 ng/mL 100 900 1000 100 ng/mL 10 990 1000 10 ng/mL 1 999 1000 1ng/mL 0 1000 1000 blank Table 1. Protocol for preparing standard curve. 3.2.3 After incubation calibrate the Picofluor Fluorometer. Press “STD VAL” and enter “999” for the highest standard ...
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AMP-Glo Assay Technical Manual TM384 - Promega
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www.promega.comTECHNICAL REFERENCE PROMEGA CORPORATION 2800 WOODS HOLLOW ROAD MADISON, WI 53711-5399 USA TELEPHONE 608-274-4330 www.promega.com ©2010 ALL RIGHTS RESERVED PART #GE647 Temperature Dependence of pH for Commonly Used Buffers
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www.promega.comThe ligation conditions given in this protocol are based on the conditions used at Promega for quality control of lambda vectors with sticky ends. These ligation condi-tions have been developed using Promega Blue/White Cloning-Qualified T4 DNA Ligase. 3. The addition of polyethylene glycol (PEG) to ligation reactions can promote ligation of
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