Transcription of Transgene Detection Primer Set for Real Time (Mouse)
1 3788 Transgene Detection Primer Setfor Real Time (Mouse) v201903Da2 3788 GFP EGFP, AcGFP1 lacZ PCR PCR DNA Ywhaz, Raver2 I 100 1 6 Primer Set ID 1 GFP_primer-1 22 M each500 l127 bp2 GFP_primer-2 32 M each500 l162 bp3 lacZ_primer-1 42 M each500 l96 bp4 lacZ_primer-2 42 M each500 l141 bp5 Reference_primer-1 52 M each500 l115 bpMA050370 76 Reference_primer-2 62 M each500 l89 bp 1 TB Green Premix Ex Taq II 25 l 2 GFP_primer-1 EGFP AcGFP1 3 GFP_primer-2 EGFP AcGFP1 4 lacZ Primer -1.
2 2 lacZ -galactosidase 5 Reference_primer-1 15 Ywhaz 6 Reference_primer-2 4 Raver2 7 Perfect Real Time Primer Set ID Perfect Real Time 8 8 Perfect Real Time RefSeq Ensembl Plants TB Green RT-PCR PCR TB Green Premix Ex Taq II (Tli RNaseH Plus) RR820S/A/B 9TB Green Premix Ex Taq (Tli RNaseH Plus) RR420S/A/B 9 ml PCR Thermal Cycler Dice Real Time System II MRQ TP960 9 PCR qPCR 2017 10 TB Green 3 3788II 20 C 2 III 1 DNA DNA NucleoSpin Tissue DNA DNA V.
3 2 PCR PCR TB Green Premix Ex Taq II (Tli RNaseH Plus) PCR Thermal Cycler Dice Real Time System II1 PCR 6 7 20 l 5 l 1 7 TB Green Premix Ex Taq II 2 lPrimer mix 2 M each 5 l- DNA 2 l14 l lTotal25 l DNA TB Green DNA 1/10 10 20 ng 2 PCR 95 C1 95 C5 40 60 C30 PCR TB Green Premix Ex Taq II 1 4 3788V DNA 1 2 3 mm ml 2 100 l NaOH solution NaOH.
4 2 mM EDTA 3 100 C 20 30 4 100 l 40 mM Tris-HCl 5 6 30 l PCR IV DNA PCR 1 EGFP lacZ DNA PCR 5 Zygosity Tg#1, Tg#3 Tg#2, Tg#4 Wt EGFP lacZ NucleoSpin Tissue DNA 1 l PCR PCR TB Green Premix Ex Taq (Tli RNaseH Plus) Multiplate RQ 2 PCR Ct 4 Tg#1 Tg#3 Tg#2 Tg#4 2 EGFP lacZ Wt EGFP lacZ GFP-1 GFP-2lacZ-1lacZ-2 Reference-1 Reference-2 Ref-2-WtRef-2-Tg #4 Ref-2-Tg #3 Ref-2-Tg #2 Ref-2-Tg #1 Ref-1-WtRef-1-Tg #4 Ref-1-Tg #1 Ref-1-Tg #2 Ref-1-Tg #3lacZ-2-WtlacZ-2-Tg #3lacZ-2-Tg #4lacZ-2-Tg #1lacZ-1-Tg #4lacZ-1-Tg #3lacZ-1-Tg #2lacZ-1-Tg #1 GFP-2-WtGFP-2-Tg #4 GFP-2-Tg #3 GFP-2-Tg #2 GFP-2-Tg #1 GFP-1-Tg #4 GFP-1-Tg #3 GFP-1-Tg #2 GFP-1-Tg #1lacZ-2-Tg #2lacZ-1-WtGFP-1-WtTarget-AssociationRel ative Quantity(2nd Derivative Max.)
5 12304v201903 DaVI NucleoSpin Tissue TB Green Premix Ex Taq II (Tli RNaseH Plus) RR820S/A/B TB Green Premix Ex Taq (Tli RNaseH Plus) RR420S/A/B Thermal Cycler Dice Real Time System II TP900 Thermal Cycler Dice Real Time System II MRQ TP960 Thermal Cycler Dice Real Time System Lite TP700 Thermal Cycler Dice Real Time System Lite MRQ TP760 MRQ Multiplate RQ TP960 TP760 Multiplate RQ PCR VII TB Green Thermal Cycler Dice Premix Ex Taq
