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Affinity Chromatography: Principles and Applications

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1 Affinity chromatography : Principles and Applications Sameh Magdeldin1,2 and Annette Moser3 1Department of Structural Pathology, Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University, 2Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 3Department of Chemistry, University of Nebraska at Kearney, Kearney, NE, 1Japan 2Egypt 3USA 1. Introduction Since the inception of Affinity chromatography 50 years ago (Cuatrecasas et al, 1968), traditional purification techniques based on pH, ionic strength, or temperature have been replaced by this sophisticated approach. It has been stated that over 60% of all purification techniques involve Affinity chromatography (Lowe, 1996).

Affinity Chromatography 8 pore size. Often, the optimal pore size takes in to account the ability of the affinity ligand to interact with the biomolecule as well as the surf ace area of the column since increasing pore

  Affinity, Chromatography, Columns, Affinity chromatography

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