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cDNA Synthesis System

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Instruction ManualcDNA Synthesis SystemCAT. NO. 18267-013Table of Contentsi1. Notices to Customer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Important Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12. Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . The cDNA Synthesis System .

the cells. This often is accomplished by using protein denaturants such as phenol, chloroform, sodium dodecyl sulfate, or chaotropic salts (9,10). The rigor of the conditions needed to prevent degradation can vary with the source of the RNA. It is also possible to introduce RNases into a preparation of RNA by using

  Phenol, Chloroform

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