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Dynamic Light Scattering Training

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Dynamic Light Scattering Training Achieving reliable nano particle sizing Dr Ryan Shaw (Sales and Application Support) Light Scattering Theory Aims of this 1 Understand what Light Scattering is 2 Understand what Rayleigh Scattering is 3 Understand what Mie Scattering is What is Light Scattering ? Light Scattering is a consequence of the interaction of Light with the electric field of a small particle or molecule An incident photon induces an oscillating dipole in the electron cloud As the dipole changes, energy is radiated in all directions This radiated energy is called scattered Light Rayleigh Theory Rayleigh theory is applicable for small particles and molecules whose diameters are less than 1/10th of the laser wavelength ( For the He-Ne laser ( = 633nm) used in the Zetasizer, this equates to particles less than about 60nm The Scattering produced by such small particles is isotropic equal in all directions The intensity of Light they produce is proportional to d6 where d is the particle diameter (ie.))

Dynamic Light Scattering and Brownian Motion › Dynamic light scattering is a non-invasive technique for measuring the size of particles and molecules in suspension › Brownian motion is the random movement of particles due to collisions caused by bombardment by the solvent molecules that surround them

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