ELISA technical guide and protocols

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TECH TIP # 65 ELISA technical guide and protocols Table of Contents Introduction ........................................ ........................................ ........................................ ........................................ .................2 Variations between ELISA protocols ........................................ ........................................ ........................................ ..................3 A. Antigen B. Direct vs. Indirect Detection of C. Biotin Signal Amplification ........................................ ........................................ ........................................ ..................3 D. Fluorescence.

Sandwich ELISAs have become very popular when using complex protein samples because only the specific antigen becomes immobilized rather than the entire sample of proteins. The more antigen that is immobilized, the higher is the potential sensitivity of the assay.

  Sandwich