Gen5 Sample Protocols and Experiments Guide

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Gen5. Sample Protocols &. Experiments Guide 2006 BioTek Table of Contents 5. Absorbance ........................................ ........................................ ............................ 7. 260/280 Ratio Test ........................................ ........................................ ............... 8. alamarblue (Absorbance) ........................................ ........................................ .... 9. BCA Protein Assay ........................................ ........................................ .............. 10. BioCell Layout protocol ........................................ ........................................ ........ 11. Bradford Protein Assay ........................................ ........................................ ........ 12. Direct dsDNA Quantitation.

of AlamarBlue results in a measurable color shift. The wavelengths for maximal absorbance are 570 nm and 600 nm for the reduced and oxidized forms of alamarBlue respectively. Because the wavelengths overlap it is necessary to measure the absorbance at both wavelengths. Protocol name: AlamarBlue-A.prt (Absorbance Readers)

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