Transcription of Gen5 Sample Protocols and Experiments Guide
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Gen5 Sample Protocols and Experiments Guide
Gen5. Sample Protocols &. Experiments Guide 2006 BioTek Table of Contents 5. Absorbance .. 7. 260/280 Ratio Test .. 8. alamarblue (Absorbance) .. 9. BCA Protein Assay .. 10. BioCell Layout protocol .. 11. Bradford Protein Assay .. 12. Direct dsDNA Quantitation .. 13. Direct Oligonucleotide Quantitation .. 14. Direct RNA 15. EDTA Inhibition Assay .. 16. IC50 (Toxicity) Assay .. 17. Kinetic Analysis -Galactosidase with ONPG 18. Kinetic Endotoxin 19. Lactate NADH Assay .. 20. Lowry Protein Assay .. 21. Particulate Test A320 .. 22. Phenol 23. Fluorescence .. 25. alamarblue (Fluorescent) .. 26. Alexa Fluor 488 .. 27. AttoPhos .. 28. Calcium Green .. 29. Caspase-3 AMC Substrate .. 30. Caspase-3 (Assay Design).. 31. Caspase-6 (Assay Design).. 32. Catalase (Amplex Red) .. 33. CBQCA .. 34. Cholesterol (Amplex Red) .. 35. CyQuant Cell Proliferation Assay .. 36.
of AlamarBlue results in a measurable color shift. The wavelengths for maximal absorbance are 570 nm and 600 nm for the reduced and oxidized forms of alamarBlue respectively. Because the wavelengths overlap it is necessary to measure the absorbance at both wavelengths. Protocol name: AlamarBlue-A.prt (Absorbance Readers)
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