Gen5 Sample Protocols and Experiments Guide

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Gen5. Sample Protocols &. Experiments Guide 2006 BioTek Table of Contents 5. Absorbance ........................................ ........................................ ............................ 7. 260/280 Ratio Test ........................................ ........................................ ............... 8. AlamarBlue (Absorbance) ........................................ ........................................ .... 9. BCA Protein Assay ........................................ ........................................ .............. 10. BioCell Layout protocol .

Experiment file name: Bradford Protein Assay with data.xpt The experiment file contains the absorbance determination, which is used to plot the standard curve. Plate Configuration: The plate layout includes standards in duplicate, from 0 to 100 µg/ml, in well positions A1 through B5.

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