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Gen5 Sample Protocols and Experiments Guide

Gen5. Sample Protocols &. Experiments Guide 2006 BioTek Table of Contents 5. Absorbance .. 7. 260/280 Ratio Test .. 8. AlamarBlue (Absorbance) .. 9. BCA Protein Assay .. 10. BioCell Layout protocol .. 11. Bradford Protein Assay .. 12. Direct dsDNA Quantitation .. 13. Direct Oligonucleotide Quantitation .. 14. Direct RNA 15. EDTA Inhibition Assay .. 16. IC50 (Toxicity) Assay .. 17. Kinetic Analysis -Galactosidase with ONPG 18. Kinetic Endotoxin 19. Lactate NADH Assay .. 20. Lowry Protein Assay .. 21. Particulate Test A320 .. 22. Phenol 23. Fluorescence .. 25. AlamarBlue (Fluorescent) .. 26. Alexa Fluor 488 .. 27. AttoPhos .. 28. Calcium Green.

Experiment file name: Bradford Protein Assay with data.xpt The experiment file contains the absorbance determination, which is used to plot the standard curve. Plate Configuration: The plate layout includes standards in duplicate, from 0 to 100 µg/ml, in well positions A1 through B5.

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