Guide to Baculovirus Expression Vector Systems (BEVS) …

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Instruction ManualGuide to BaculovirusExpression Vector Systems (BEVS) and Insect Cell CultureTechniquesINSIDE FRONT of Baculovirology ........................................ ........................................ ........................................ .................... 1 Baculoviruses as Expression Vectors ........................................ ........................................ ........................................ .... 1Advantages of BEVS 2Generating a Recombinant Virus by Homologous Recombination ........................................ ........................................ .3Generating a Recombinant Virus by Site-Specific Transposition ........................................ ........................................ ....3Insect Cell Culture for Culturing Host 6Protocol 1:Subculturing Monolayer 6Protocol 2:Adapting Monolayer Cells to Suspension Culture.

under phase contrast microscopy. Infected cultures stop growing. 3. Very Late Phase:Within 20 to 36 h after infection, cells cease production of budded virus and begin the assem-FIGURE 1. In vivobaculovirus infection and replication. Insects killed by a baculo-virus infection release poly-hedra onto plant surfaces.

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