Guide to Baculovirus Expression Vector Systems (BEVS) …

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Instruction ManualGuide to BaculovirusExpression Vector Systems (BEVS) and Insect Cell CultureTechniquesINSIDE FRONT of Baculovirology ........................................ ........................................ ........................................ .................... 1 Baculoviruses as Expression Vectors ........................................ ........................................ ........................................ .... 1Advantages of BEVS 2Generating a Recombinant Virus by Homologous Recombination ........................................ ........................................ .3Generating a Recombinant Virus by Site-Specific Transposition ........................................ ........................................ ....3Insect Cell Culture for Culturing Host 6Protocol 1:Subculturing Monolayer 6Protocol 2:Adapting Monolayer Cells to Suspension Culture.

villar epithelial cells. Infected cells release EV from the basement membrane side of the mid-gut cell into the hemolymph system. Horizontal Transmission EV enters the insect hemocoel and immediately spreads throughout the insect’s open circulatory system, infecting many cell types. Within 10 viral generations, the insect dies

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