Guide to Baculovirus Expression Vector Systems (BEVS) …

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Instruction ManualGuide to BaculovirusExpression Vector Systems (BEVS) and Insect Cell CultureTechniquesINSIDE FRONT of Baculovirology ........................................ ........................................ ........................................ .................... 1 Baculoviruses as Expression Vectors ........................................ ........................................ ........................................ .... 1Advantages of BEVS 2Generating a Recombinant Virus by Homologous Recombination ........................................ ........................................ .3Generating a Recombinant Virus by Site-Specific Transposition ........................................ ........................................ ....3Insect Cell Culture for Culturing Host 6Protocol 1:Subculturing Monolayer 6Protocol 2:Adapting Monolayer Cells to Suspension Culture.

affect the insect population. They are double-stranded, circular, supercoiled DNA molecules in a rod-shaped capsid (1). More than 500 baculovirus isolates (based on hosts of origin) have been identified, most of which originated in arthropods, particularly insects of the order Lepidoptera (2,3). Two of the most common isolates used in foreign

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