Relative quantification
Relative quantificationMichael W. Pfaffl in: Real-time PCR. Published by International University Line (Editor: T. Dorak), p IntroductionReverse transcription (RT) followed by a polymerase chain reaction (PCR)represents the most powerful technology to amplify and detect traceamounts of mRNA (Heid et al., 1996; Lockey, 1998). To quantify these lowabundant expressed genes in any biological matrix the real-time quantita -tive RT-PCR (qRT-PCR) is the method of choice. Real-time qRT-PCR hasadvantages compared with conventionally performed semi-quantitativeend point RT-PCR, because of its high sensitivity, high specificity, goodreproducibility, and wide dynamic quantification range (Higuchi et al.)
understanding of its underlying quantification principles is important. ... This chapter describes the relative quantification strategies in quantita-tive real-time RT-PCR with a special focus of relative quantification models and newly developed relative quantification software tools.
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