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Troubleshooting Common HPLC Problems

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Troubleshooting Common hplc ProblemsWaters Corp. 2002Potential Sources of Chromatographic ProblemszMobile PhasezInjectorzIn-Line FilterzColumnzDetectorzSamplezPumpzGuard ColumnzConnecting Tubing and FittingszIntegrator/RecorderSoftwareScie ntist/Analyst --need for logical approach to save timeWaters Corp. 2002ProblemCHEMISTRYzCOLUMNzGUARD COLUMNzMOBILE PHASEzSAMPLE/VIALSzPUMPzINJECTORzDETECTO RzDATA COLLECTIONzBAND SPREADING/CONNECTIONSzCOLUMNSzSAMPLE VIALSTroubleshooting StrategyTry to simplify --assess impact on lab efficiency --inspect the chromatography --try to categorize troubleshoot the easiest to fix items firstMECHANICALWaters Corp. 2002Detector CellWaters c 1998How to Categorize --Inspect Chromatogram How do you get sharp peaks with excellentresolution?

Retention Time Variability. Less or More Retention Time . All Peaks • Pump Flow Rate Problem (check actual volume/time being delivered) • Wrong Column Type (C8 – less retention, vs C18 – more retention) • Temperature Problem (warmer – less retention, colder – more retention) • % Organic In Mobile Phase (more organic – less ...

  Troubleshooting, Retention, Problem, Common, Hplc, Columns, Troubleshooting common hplc problems

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