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Troubleshooting Common HPLC Problems

Troubleshooting Common hplc ProblemsWaters Corp. 2002 Potential Sources of Chromatographic ProblemszMobile PhasezInjectorzIn-Line FilterzColumnzDetectorzSamplezPumpzGuard ColumnzConnecting Tubing and FittingszIntegrator/RecorderSoftwareScie ntist/Analyst --need for logical approach to save timeWaters Corp. 2002 ProblemCHEMISTRYzCOLUMNzGUARD COLUMNzMOBILE PHASEzSAMPLE/VIALSzPUMPzINJECTORzDETECTO RzDATA COLLECTIONzBAND SPREADING/CONNECTIONSzCOLUMNSzSAMPLE VIALST roubleshooting StrategyTry to simplify --assess impact on lab efficiency --inspect the chromatography --try to categorize troubleshoot the easiest to fix items firstMECHANICALW aters Corp.

Retention Time Variability. Less or More Retention Time . All Peaks • Pump Flow Rate Problem (check actual volume/time being delivered) • Wrong Column Type (C8 – less retention, vs C18 – more retention) • Temperature Problem (warmer – less retention, colder – more retention) • % Organic In Mobile Phase (more organic – less ...

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