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Troubleshooting Common HPLC Problems

Retention Time Variability. Less or More Retention Time . All Peaks • Pump Flow Rate Problem (check actual volume/time being delivered) • Wrong Column Type (C8 – less retention, vs C18 – more retention) • Temperature Problem (warmer – less retention, colder – more retention) • % Organic In Mobile Phase (more organic – less ...

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Clinical Chemistry - Mohsin Al-Saleh

mohsinalsaleh.com

vein (in the arm) directly into an evacuated tube. Typically a tube will hold about 5 mL of blood – enough to perform many clinical chemistry tests since automated analyzers require only small amounts (typically from 2 to 100 μL) for a single test. Occasionally, when collection of blood from a vein is difficult, a sample of

  Chemistry, Clinical, Tubes, Evacuated, Clinical chemistry, Evacuated tube

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The Analysis of a Broad Range of Organic Acids by HPLC ...

resources.perkinelmer.com

System was used, including the Altus A-10 Solvent/Sample Module, integrated vacuum degasser, A-10 column heater, and Altus A-10 UV detector. All instrument control, analysis and data processing was performed using the Waters ® Empower 3 Chromatography Data Software (CDS) platform. Method Parameters. The LC method parameters are shown in Table 1.

  Data, Ranges, Board, Acid, Organic, Solvents, Columns, Broad range of organic acids

HYDROCARBONS, BP 36°-216 °C 1500

www.cdc.gov

Analy te Approximate Retention Time (min) n-pent ane 7.5 solvent (CS 2) 9.6 n-hexane 13.0 cycl ohexane 16.1 cycl ohexene 16.8 n-hept ane 17.7 methyl cycl ohexane 18.9 n-oct ane 21.6 n-nonane 24.9 n-decane 27.8 n-undecane 30.5 n-dodecane 32.9 NOTE: Retenti on times may vary slightly due to column manufacturer and age of column, and be

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Fundamentals of HPLC

polymer.ustc.edu.cn

• Frequently called the data system, the computer not only controls all the modules of the HPLC instrument but it takes the signal from the detector and uses it to determine the time of elution (retention time) of the sample components (qualitative analysis) and the amount of sample (quantitative analysis). 1 2 4 5 Solvent reservoirs and ...

  Data, Retention, Fundamentals, Hplc, Solvents, Fundamentals of hplc

METHANOL 2000

www.cdc.gov

However, any compound with a similiar retention time may interfere. Positive identification can be confirmed by dual column chromatography with a 100% PEG capillary column. Mass spectrometry can also be used as a confirmation aid. OTHER METHODS: This method updates and replaces NMAM 2000 (August 15, 1994), which combined and replaced Method S59

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Boeing Process Specification Index - Moog Inc.

www.moog.com

Listing (QPL) the revision status of this is given in the PSD column. Items marked with a have been updated since the last revision. 4.2 In the column “Date of most recent update” a date of 7-Aug-18 represents the date at which the initial issue status data was gathered for this document. Additionally, the date given is

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SIZE EXCLUSION CHROMATOGRAPHY FOR BIOMOLECULE …

www.agilent.com

Column Selection Column size SEC columns are usually much larger than those used for other types of chromatography and are operated at comparatively low flow rates or slow linear velocities. The standard column dimensions for SEC are 7.8 x 300 mm, operated at 1.0 mL/min, compared to a reversed-phase column that is likely to be 2.1 or 4.6 x 150 ...

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Broad Range of Organic Acids, Solvent, Column, Data, Retention, Retenti on, Fundamentals of HPLC, METHANOL 2000, Process Specification, SIZE EXCLUSION CHROMATOGRAPHY FOR BIOMOLECULE