Exclusion Chromatography
Found 8 free book(s)Size Exclusion Chromatography
wolfson.huji.ac.ilSize Size exclusion chromatography (SEC), also called gel filtration (GF) Hydrophobicity Hydrophobic interaction chromatography (HIC) Reversed phase chromatography (RPC) Charge Ion exchange chromatography (IEX) Biorecognition (ligand specificity) Affinity chromatography (AC) Isoelectric point (pI) Chromatofocusing (CF) Fig I.1.
Size Exclusion Chromatography Separations Module
asdlib.orgSize Exclusion Chromatography (SEC) Separations Module Goal: This module is designed to provide theory and practical use of size exclusion chromatography (SEC) for separation of proteins. In-Class Questions: 1. What is an obvious physical difference between an …
GE Healthcare - Fred Hutch
research.fredhutch.orgCharge Ion exchange chromatography (IEX) Size Size exclusion chromatography (SEC), also called gel filtration (GF) Hydrophobicity Hydrophobic interaction chromatography (HIC) Reversed phase chromatography (RPC) Biorecognition (ligand specificity) Affinity chromatography (AC) Fig I.1. Separation principles in chromatographic purification.
Basic Principles of Chromatography
anuragaja.staff.ipb.ac.idSize-exclusion chromatography Liquid/solid Molecular size Hydrophobic-interaction chromatography Polar liquid/nonpolar liquid or solid Molecular size/polarity Affinity chromatography Water/binding sites Specific structure Reprinted from ( 8), p. A21, with kind permission from Elsevier Science-NL, Sara Burgerhartstraat 25, 1055KV Amsterdam,
SIZE EXCLUSION CHROMATOGRAPHY FOR BIOMOLECULE …
www.agilent.comSize exclusion chromatography is suitable for separating and quantifying protein mixtures, and is therefore a valuable technique for quality control in recombinant protein manufacture. This includes measuring aggregates (dimers, trimers, tetramers, etc.) or separating low molecular weight excipients and impurities
HPLC Purification of mRNA with Reverse Phase and …
www.nacalai.co.jpsize exclusion chromatography (SEC). mRNA ranging in size from 1100-1950 nt were purified with a column containing particles with 1000Å pore size. Good peak shape was obtained using a mobile phase of 100mM triethylammonium acetate (TEAA) and acetonitrile (A). Higher molecular weight impurities, potentially template DNA were seen before the main
Introduction to Liquid Chromatography
www.chem.tamu.eduChromatography (TLC) by Kirchner in the U.S. 1952: Martin and Synge receive Nobel Prize for “invention of partition chromatography” or plate theory to describe column efficiency 1966: HPLC was first named by Horvath at Yale University but HPLC didn’t “catch on” until the 1970s 1978: W.C. Stills introduced “flash chromatography”,
NGC Chromatography Systems - Bio-Rad
www.bio-rad.comNGC Medium-Pressure Chromatography Systems The NGC instrument is an automated liquid chromatography system focused on biomolecule purification at the research, process development, and laboratory-scale levels. At the core of the NGC platform is a truly customizable and scalable system