Search results with tag "Exclusion chromatography"
Size Exclusion Chromatography
wolfson.huji.ac.ilSize Size exclusion chromatography (SEC), also called gel filtration (GF) Hydrophobicity Hydrophobic interaction chromatography (HIC) Reversed phase chromatography (RPC) Charge Ion exchange chromatography (IEX) Biorecognition (ligand specificity) Affinity chromatography (AC) Isoelectric point (pI) Chromatofocusing (CF) Fig I.1.
GE Healthcare - Fred Hutch
research.fredhutch.orgCharge Ion exchange chromatography (IEX) Size Size exclusion chromatography (SEC), also called gel filtration (GF) Hydrophobicity Hydrophobic interaction chromatography (HIC) Reversed phase chromatography (RPC) Biorecognition (ligand specificity) Affinity chromatography (AC) Fig I.1. Separation principles in chromatographic purification.
Size Exclusion Chromatography Separations Module
asdlib.orgSize Exclusion Chromatography (SEC) Separations Module Goal: This module is designed to provide theory and practical use of size exclusion chromatography (SEC) for separation of proteins. In-Class Questions: 1. What is an obvious physical difference between an …
Basic Principles of Chromatography
anuragaja.staff.ipb.ac.idSize-exclusion chromatography Liquid/solid Molecular size Hydrophobic-interaction chromatography Polar liquid/nonpolar liquid or solid Molecular size/polarity Affinity chromatography Water/binding sites Specific structure Reprinted from ( 8), p. A21, with kind permission from Elsevier Science-NL, Sara Burgerhartstraat 25, 1055KV Amsterdam,
Types of liquid chromatography - ut
tera.chem.ut.ee1 1 Types of liquid chromatography 2 3 We focus on the stationary phase chemistry: • Normal and reversed phase – Ion-pair chromatography • Size exclusion chromatography
Shimadzuʼs Fundamental Guide to Gas Chromatography …
www.shimadzu.eu.comSEC Size Exclusion Chromatography SIM Selected Ion Monitoring SRM Selected Reaction Monitoring TIC Total Ion Current TOF Time-of-Flight TQ Triple Quadrupole UFMS Ultra-Fast Mass Spectrometry UHPLC Ultra-High-Performance Liquid Chromatography UV-VIS Ultraviolet-Visible Spectroscopy 6. Symbols. µ Micro amu Atomic mass unit ...
SIZE EXCLUSION CHROMATOGRAPHY FOR BIOMOLECULE …
www.agilent.comSize exclusion chromatography is suitable for separating and quantifying protein mixtures, and is therefore a valuable technique for quality control in recombinant protein manufacture. This includes measuring aggregates (dimers, trimers, tetramers, etc.) or separating low molecular weight excipients and impurities
HPLC Purification of mRNA with Reverse Phase and …
www.nacalai.co.jpsize exclusion chromatography (SEC). mRNA ranging in size from 1100-1950 nt were purified with a column containing particles with 1000Å pore size. Good peak shape was obtained using a mobile phase of 100mM triethylammonium acetate (TEAA) and acetonitrile (A). Higher molecular weight impurities, potentially template DNA were seen before the main
Waters 2414 Refractive Index Detector
www.waters.comanalyses by gel-permeation or size-exclusion chromatography. RI detectors work by measuring changes in the bulk properties of the solvent and the solutes under investigation, exploiting the improbability that solvents . Refractive index Refractive index) = …