Peptone
Found 8 free book(s)Types of culture media used in microbiology
www.ramauniversity.ac.inPeptone Water. Peptone 1% and sodium chloride 0.5%. It is used as base for sugar media and to test indole formation. Blood Agar. Most commonly used medium. 5- 10% defibrinated sheep or horse blood is added to melted agar at 45-50°C. Blood acts …
Nutrient Agar - himedialabs.com
himedialabs.comPeptone, HM peptone B and yeast extract provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria. Sodium chloride maintains the osmotic equilibrium of the medium. # - Equivalent to Beef extract Type of specimen
PREPARATION OF MEDIA I. OBJECTIVES II. INTRODUCTION
www.sas.upenn.eduPeptone is used in culture media to mainly supply nitrogen. Most organisms are capable of utilizing the amino acids and other simpler nitrogenous compounds present in peptone. Thus, in many cases, the complicated infusion media can be replaced by simpler media prepared by
Urease Test Protocol
asm.orgPeptone 1 g Dextrose 1 g Sodium chloride 5 g Potassium phosphate, monobasic 2 g Urea 20 g Phenol red 0.012 g Agar 15 to 20 g To prepare the urea base, dissolve the first six ingredients in 100 ml of distilled water and filter sterilize (0.45-mm pore size). Suspend the agar
Product Instructions
multimedia.3m.com(buffered peptone water (BPW) (ISO)) may inhibit the DNase reaction resulting in no pink zone formation when the 3M Petrifilm STX Plate is used with the 3M Petrifilm STX Disk. It is important to verify the performance of the 3M Petrifilm STX Disk with the diluent chosen for sample preparation. Failure to do so, may result in false negatives.
LAB 4. Cultivation of Bacteria - University of Maryland ...
science.umd.eduPeptone 5.0g Beef extract 3.0 g Sodium Chloride 8.0 g Agar 15.0 g Water 1 1iter Glucose Minimal Salts Broth: Example of chemically defined medium designed for the growth of E.coli. Constituent Amount Glucose 5.0 g Ammonium phosphate monobasic 1.0 g Sodium chloride 5.0 g Magnesium sulfate 0.2 g Potassium phosphate, dibasic 1.0g Water 1 liter
2.6.12. MICROBIOLOGICAL EXAMINATION OF NON-STERILE ...
uk.vwr-cmd.comchloride-peptone solution pH 7.0 or in another suitable liquid. In general a one in ten dilution is prepared. However, the characteristics of the product or the required sensitivity may necessitate the use of other ratios. If the product is known to have antimicrobial activity, an inactivating agent may be added to the diluent.
3.8 Bacterial Identification Techniques - FWS
www.fws.govprotein (peptone) present in the agar. In this case, nitrogenous bi-products are produced and the pH in the media rises until the pH indicator shows a reversion from yellow (acid) to red (alkaline). Blackened medium is caused by hydrogen sulfide production, which changes ferrous sulfate to …