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PROTOCOL PowerUp SYBR Green Master Mix Guidelines for ...

tools.thermofisher.com

Real-time PCR 1. Place the plate in the instrument. 2. Set the thermal cycling conditions. ... † For 7500 Fast, StepOne, or StepOnePlus instruments. ‡ For primers with T m <60°C, please refer to the PowerUp SYBR Green Master Mix instruction manual. • ™Include conditions for melt curve analysis after the PCR stage. On Applied Biosystems ...

  Time, 7500, Master, Real, Green, Time pcr, Fast, Sybr, Sybr green master mix, 7500 fast

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