Transcription of 2. TCA/Acetone Protein Precipitation Protocol
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2. TCA/Acetone Protein Precipitation Protocol
Proteomics and Mass Spectrometry Facility, BRC, Cornell University Version 2. TCA/Acetone Protein Precipitation Protocol The following procedure is used to precipitate proteins from freshly prepared cell lysates containing some contaminants, such as salt, detergents, nucleic acids, and lipids that can interfere with the subsequent 2D analysis. TCA/Acetone Precipitation is one of the most common Precipitation methods. The acetone used in this procedure should be pre-chilled and stored in a -20 C freezer until needed and kept on ice during the entire procedure. Procedure 1. Mix the following solutions in a 1:8:1 ratio in the following order (invert tube after adding each component): 1 ml cell lysate 8 ml 100% ice-cold acetone 1 ml 100% trichloroacetic acid (100%TCA, w/v) 2.
TCA/Acetone Protein Precipitation Protocol The following procedure is used to precipitate proteins from freshly prepared cell lysates containing some contaminants, such as …
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