Transcription of A Guide to HPLC and LC-MS Buffer Selection
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A Guide to HPLC and LC-MS Buffer Selection
A Guide to HPLC. and LC-MS . Buffer Selection HPLC Columns John Dolan Your decision has lasting effects. Choose wisely. HPLC Columns Ultra Inert Base-Deactivated HPLC Columns For Performance, Selectivity and Guaranteed Reproducibility . performance ACE guarantee If ACE does not outperform your existing column (of equivalent phase, particle size and dimensions), send in your comparative data within 60. days and keep the ACE column FREE OF CHARGE. Using Buffers with HPLC and LC-MS . Table of Contents Page Why Control pH? 2. Practical Implications 4. Controlling pH 4. What about LC-MS ? 6. Just Interested in Low pH? 7. Or High pH? 7. How Much? 7. Solubility Issues 10. Dilution Effects 11. Buffer Preparation 12. Precautions 13. Summary 15. References 16. Using Buffers with HPLC and LC-MS .
www.ace-hplc.com 5 additives are listed in Table 1. A buffer is most effective when used within ±1 pH unit of its pKa, but may provide adequate buffering ±2 pH units from the pKa. Table 1.pKa Values of Common Mobile Phase Additives1 The most popular buffers for HPLC with UV detection are phosphate and
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