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Analysis of Cell Cycle by Flow Cytometry

Analysis of Cell Cycle by Flow Cytometry 301. 22. Analysis of Cell Cycle by Flow Cytometry Piotr Pozarowski and Zbigniew Darzynkiewicz Summary Described are four widely used procedures to analyze the cell Cycle by flow Cytometry . The first two are based on univariate Analysis of cellular DNA content following cell staining with either propidium iodide (PI) or 4',6'-diamidino-2-phenylindole (DAPI) and deconvolution of the cellular DNA content frequency histograms. This approach reveals distribution of cells in three major phases of the Cycle (G1 vs S vs G2/M) and makes it possible to detect apoptotic cells with fractional DNA content. The third approach is based on the bivariate Analysis of DNA.

Introduction A variety of flow or cytometric methods to analyze the cell cycle progres-sion have been developed over the past three decades. These methods can be grouped into three categories: 1. In the first are the methods that rely on a single time point (“snapshot”) cell measurement. This analysis may be either univariate, generally ...

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