Transcription of Analyzing Differences in Bacterial Optical Density ...
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Analyzing Differences in Bacterial Optical Density ...
Analyzing Differences in Bacterial Optical Density Measurements between SpectrophotometersBrian C. Matlock1, Richard W. Beringer1, David L. Ash1, Michael W. Allen2 and Andrew F. Page11 ThermoFisher Scientific, UV-Vis/Fluor-Molecular Spectroscopy, Wilmington, DE. 2 ThermoFisher Scientific, UV-Vis/Fluor-Molecular Spectroscopy, Madison, Density (OD) measurement of Bacterial cultures is a common technique used in microbiology. Researchers have primarily relied on spectrophotometers to make these measurements, however the measurement is actually based on the amount of light scattered by the culture rather than the amount of light absorbed.
Viable Counts: At each 30-minute interval, an aliquot of the sample was also used to perform serial dilutions. Dilutions were plated on LB Agar plates and incubated at 37°C overnight. Colonies were counted to determine bacterial cell count (CFU/ml) at each time point.
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