Annex 5 Multiple-tube method for thermotolerant (faecal ...

Annex 5189 Annex 5 Multiple-tube method forthermotolerant (faecal) coliformsIn the Multiple-tube method , a series of tubes containing a suitable selective brothculture medium is inoculated with test portions of a water sample. After aspecified incubation time at a given temperature, each tube showing gas forma-tion is regarded as presumptive positive since the gas indicates the possiblepresence of coliforms. However, gas may also be produced by other organisms,and so a subsequent confirmatory test is essential. The two tests are knownrespectively as the presumptive test and the confirmatory the confirmatory test, a more selective culture medium is inoculated withmaterial taken from the positive tubes . After an appropriate incubation time, thetubes are examined for gas formation as before.

ANNEX 5 189 Annex 5 Multiple-tube method for thermotolerant (faecal) coliforms In the multiple-tube method, a series of tubes containing a suitable selective broth

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  Multiple, Methods, Tubes, Coliform, Thermotolerant, Alface, Multiple tube method for thermotolerant

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