Transcription of Cell culture guidelines - abcam.com
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Cell culture guidelines - abcam.com
Discover more at Cell culture guidelines The following is a general guideline for culturing of cell lines. All cell culture must be undertaken in microbiological safety cabinet using aseptic technique to ensure sterility. 1. Preparing an aseptic environment 1. Hood regulations (a) Close hood sash to proper position to maintain laminar air flow (b) Avoid cluttering 2. Autoclaving (a) Pipette tips (or can be purchases pre-autoclaved, DNAse/RNAse free) (b) Glass 9 Pasteur pipettes (c) 70% ethanol. Be sure to spray all surface areas All media, supplement and reagents must be sterile to prevent microbial growth in the cell culture . Some reagents and supplements will require filter sterilization if they are not provided sterile.
Discover more at abcam.com/technical 1. Prepare 10mL of coating solution composed of 1% gelatin or 1% fibronectin by diluting with distilled water followed by filtration.
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