Biology of Fungi, Lecture 3: Fungal Structure and …

Biology of Fungi Lecture 3: Fungal Structure and Function Page 2 of 5 Fungal Cell Wall u Functions Q Structural barrier Q Determines pattern of cell …

  Structure, Functions, Cells, Structure and function, Fungi, Structure and

GELATIN HYDROLYSIS TEST

2) Using aseptic technique and a microbiology needle, inoculate the respective tube with the appropriate bacterium by stabbing the medium three-fourths of the way to the bottom of the tube. 3) Incubate all tubes for 1 to 7 days at 37°C. 4) Periodically throughout the incubation period, remove the tubes from the incubator and

  Technique, Aseptic, Incubation, Aseptic technique

BASIC CULTURE TECHNIQUE STREAK PLATE

3) Using aseptic technique, sterilize a microbiological loop using either the gas burner or the Bacti-Cinerator (see the exercise entitled Basic Microbiology Technique: Aseptic Transfer). 4) While holding the loop between the thumb and forefinger, grasp the mixed bacterial culture in …

  Exercise, Plate, Technique, Aseptic, Aseptic technique, Starke, Technique streak plate

SELECTIVE AND DIFFERENTIAL MEDIA

as glucose-salts, as well as rich, chemically complex media such as brain heart infusion agar and tryptic soy broth. However, these general growth media may permit the growth unwanted microorganisms from clinical or environmental samples that are not relevant to the purpose of the microbiological examination.

  Agar, Selective, Differential

EFFECTS OF PH ON MICROBIAL GROWTH

• Sabouraud dextrose agar culture (3-5 days old) of Saccharomyces kudriavzevii (ATCC 2601; formerly designated as Saccharomyces cerevisiae) [abbreviated as S. kudriavzevii] Materials • Sterile saline (5 ml) in standard test tube • Sterile bulb transfer pipettes • TSB (3 ml) in 13 x 100 mm tubes • TSB, pH 3.0, (3 ml) in 13 x 100 mm tubes

  Agar, Growth, Effect, Microbial, Dextrose, Dextrose agar, Effects of ph on microbial growth

SULFIDE-INDOLE-MOTILITY (SIM) TEST

Finally, indole production is detected in SIM medium due to the presence of casein peptone, an ingredient rich in tryptophan. Organisms that possess the enzyme tryptophanase degrade tryptophan to generate indole, pyruvic acid, and ammonia. The latter two are used for a cell’s nutritional needs, whereas indole is not and accumulates in the medium.

  Lodine

CARBOHYDRATE FERMENTATION TEST - Youngstown State …

Microbiology Laboratory (BIOL 3702L) Page 1 of 6 CARBOHYDRATE FERMENTATION TEST Principle and Purpose Microorganisms need to generate energy in order to grow, divide, and survive. In any given environment, different sugars (i.e., carbohydrates) may be available. Microbes must possess the

  Microbiology, Boil, Fermentation

EFFECTS OF TEMPERATURE ON MICROBIAL GROWTH

aureus, and Pseudomonas aeruginosa. The degree to which temperature affects the growth of these microbes shall be Figure 1. Growth rates of microbes as a function of temperature. Microbes can be differentiated based upon their ranges of temperature tolerance.

  Growth, Temperatures, Microbial, Microbial growth, Pseudomonas, Pseudomonas aeruginosa, Aeruginosa

A theoretical and practical guide for spectrophotometric ...

1. Basis for spectrophotometric quantitation of proteins at 280 nm Direct spectrophotometric determination of proteins can be done at either 280 nm or 205 nm. In this note, only measurement at 280 nm will be discussed. At this wavelength, the aromatic amino acids tryptophan (Trp) and tyrosine (Tyr) exhibit strong light absorption, and to

  Spectrophotometric

EXPERIMENT 7 Spectrophotometric Iron Analysis

spectrophotometric experiment. Consider a sample of some solution contained in a small transparent vessel - perhaps a test tube. (When employed in spectrophotometric measurements the container is called a cuvet.) Imagine a beam of monochromatic light (light of a single wavelength - in practice light with a

  Spectrophotometric

Interpreting Nanodrop (Spectrophotometric) Results

Interpreting Nanodrop (Spectrophotometric) Results Foundation of Spectrophotometry: The Beer‐Lambert Law—what does it mean? A = ɛcl Where A=absorbance, ԑ=extinction coefficient, c=concentration and l=path length. The Beer‐Lambert law draws a direct correlation between absorbance and concentration.

  Interpreting, Results, Spectrophotometric, Nanodrop, Interpreting nanodrop

Lab 2 Spectrophotometric Measurement of Glucose

Spectrophotometric Technique (See Fig 2-2) 1. Plug in the spectrophotometer and turn it on by turning the left dial clockwise. Allow the spectrophotometer to warm up at least 5 minutes before proceeding. 2. Set the wavelength dial to 600 nm. This is a wavelength of light that gets absorbed readily by the pigment in the Grape Kool-Aid. 3.

  Measurement, Glucose, Spectrophotometric, Lab 2 spectrophotometric measurement of glucose

260/280 and 260/230 Ratios - CGIAR

* William W. Wilfinger, Karol Mackey, and Piotr Chomczynski, Effect of pH and Ionic Strength on the Spectrophotometric Assessment of Nucleic Acid Pu- rity: BioTechniques 22:474-481 (March 1997) • Wavelength Accuracy Of The Spectrophotometers

  Spectrophotometric

Spectrophotometric Nucleic Acid Quantification - BioTek

we’ll discuss in detail the spectrophotometric methodology as performed in microplate instrumentation and in specialized micro-volume accessories. Absorbance Quantification Methods Nucleic acid quantification is commonly performed in …

  Acid, Quantification, Nucleic, Spectrophotometric, Spectrophotometric nucleic acid quantification

Spectrophotometric Determination of Phosphates in Water

Spectrophotometric Determination of Phosphates in Water Background Controlled concentration of phosphorus in water is critical for a stable ecosystem. Aquatic plants are depen-dent on a certain amount of phosphorus in order to survive; however, excess phosphorus leads to eutroph-ication, the over enrichment of a water body with nutrients.

  Determination, Phosphate, Spectrophotometric, Spectrophotometric determination of phosphates in

Spectrophotometric Determination of an Equilibrium ...

Spectrophotometric Determination of an Equilibrium Constant v021214 Objective To determine the equilibrium constant (K c) for the reaction of iron (III) ion with thiocyanate (SCN-) to form the thiocyanatoiron(III) complex ion (FeSCN2+).This measurement is done by

  Spectrophotometric

TR0006.4 Introduction - Thermo Fisher Scientific

Pierce Biotechnology PO Box 117 (815) 968 -0747 www.thermo.com/pierce 3747 N. Meridian Road Rockford, lL 61105 USA (815) 968 -7316 fax . 2