Transcription of Development and Validation of a Reversed-Phase HPLC …
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Development and Validation of a Reversed-Phase HPLC …
ACTA CHROMATOGRAPHICA, NO. 18, 2007 Development AND Validation OF A Reversed-Phase HPLC METHOD FOR simultaneous DETERMINATION OF DOMPERIDONE AND PANTOPRAZOLE IN PHARMACEUTICAL DOSAGE FORMS T. Sivakumar*, R. Manavalan, and K. Valliappan Department of Pharmacy, Annamalai University, Annamalai Nagar, Tamil Nadu-608 002, India SUMMARY A simple Reversed-Phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of domperidone and pantoprazole in capsules. The com-pounds were separated on an ODS analytical column with a mixture of methanol, acetonitrile, and triethylamine solution (10 mM, pH adjusted with 85% phosphoric acid) in the ratio 20:33:47 (v/v) as mobile phase at a flow rate of mL min 1.
develop a suitable analytical method for simultaneous estimation of DP and PP in pharmaceutical preparations. Because HPLC methods have been widely used …
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