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Development and validation of an HPLC method …
*Correspondence: R. M. Mainardes. Departamento de Farm cia, Universidade Estadual do Centro-Oeste/UNICENTRO. Rua Sime o Camargo Varela de S 03, 85040-080 - Guarapuava - PR, Brasil. E-mail: Journal of Pharmaceutical Sciencesvol. 49, n. 1, , 2013 Development and validation of an hplc method for the determination of fluorouracil in polymeric nanoparticlesAna Cristina de Mattos, Najeh Maissar Khalil, Rubiana Mara Mainardes*Department of Pharmacy, Midwestern State University/UNICENTRO, Guarapuava, Paran , BrasilThe objective of this work was to develop and validate a rapid high performance liquid chromatography ( hplc ) method for the quantitative analysis of fluorouracil (5-FU) in polymeric nanoparticles. Chromatographic analyses were performed on an RP C18 column with a mobile phase consisting of acetonitrile and water (10:90, v/v) at a flow rate of 1 mL/min. The 5-FU was detected and quantitated using a photodiode array detector at a wavelength of 265 nm.
Development and validation of an HPLC method for the determination of fluorouracil in polymeric nanoparticles 119 method. The samples were appropriately diluted in water.
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