Transcription of EasySelect Pichia Expression Kit
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EasySelect Pichia Expression Kit
User Manual EasySelect Pichia Expression Kit For Expression of Recombinant Proteins Using pPICZ and pPICZ in Pichia pastoris Cat. no. K1740-01 Rev. Date 18 June 2010 Manual part no. 25-0172 MAN0000042 ii iii Table of Contents Important Accessory viii Introduction ..1 Overview ..1 Experimental Methods .. 7 Pichia E. coli Selecting a Pichia Expression General Cloning Cloning into Cloning into pPICZ ..21 Transformation into E. Preparing Transforming Electroporation of EasyComp Determining the Mut Expressing Recombinant Pichia Analysis by SDS-Polyacrylamide Gel Optimizing Pichia Protein Scale-up of Protein Appendix ..53 E. coli Media Recipes ..53 Pichia Media Proteins Expressed in Recombination and Integration in Lithium Chloride Transformation Zeocin.
Saccharomyces cerevisiae. It is faster, easier, and less expensive to use than other eukaryotic expression systems and generally gives higher expression levels. As a yeast, it shares the advantages of molecular and genetic manipulations with Saccharomyces, and it has the added advantage of 10- to 100-fold higher
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