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Experiment 7: Preparation of a Buffer

Experiment 7: Preparation of a BufferCH2250: Techniques in Laboratory Chemistry, Plymouth State UniversityCreated by Jeremiah Duncan and Wavell Fogleman, Department of Atmospheric Science and Chemistry, Plymouth State : The Preparation of Buffer solutions is a common task in the lab, especially in biological sciences. A Buffer is a solution that resists a change in pH, because it contains species in solution able to react with any added acid or base, according to the principles of equilibrium. You will study more about equilibrium and buffers in General Chemistry II; here we will limit ourselves to the practical matter of preparing a base at a desired pH.

Second, you will make 100 mL of a buffer also with pH = 5, but with a higher buffering capacity, using 5 mL of a 0.5 M acetic acid solution. Although a buffer will resist a change in pH, eventually enough acid or base can be added to destroy it. The amount of acid or base needed to change the pH of a buffer is known as the "buffering capacity."

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