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Fluorescence Lifetime Spectroscopy and Imaging

22 Anand T. N. Kumar22 .1 INTRODUCTIONF luorescence Lifetime Imaging microscopy (FLIM) is a well-established technique (Bastiaens and Squire 1999; Berezovska et al. 2003; Selvin 2000; Vogel et al. 2006) that combines microscopic techniques with time-resolved detection to provide high-resolution Lifetime images of thin tissue sections. This chapter concerns the in vivo time domain Imaging of fluorescent contrast agents embedded in deep tissue. Optical molecular Imaging is a rapidly growing field of interest (Bremer et al.)

22.1 Introduction 461 22.2 Theory 462 22.2.1 General forward problem statement 462 22.2.2 Frequency domain 463 ... Fluorescence lifetime imaging based on exogenous probes 464 real part of F(r,ω), respectively. As is clear from Equation 22.5, the TD data provide multiple frequencies.

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