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Fluorescence Microscopy

Fluorescence Microscopy Kenneth R. Spring National Institutes of Health, Bethesda, Maryland, introduction fluorochromes (also called fluorophores), which are ex- cited by specific wavelength irradiating light and emit When organic or inorganic specimens absorb and sub- light of useful intensity. Fluorochromes are stains that sequently reradiate light, the process is typically a result attach themselves to visible or subvisible structures, are of Fluorescence or phosphorescence. Fluorescence emis- often highly specific in their attachment targeting, and sion is nearly simultaneous with the absorption of the have significant quantum yield (the photon emission/ab- excitation light as the time delay between photon ab- sorption ratio).

Fluorescence Microscopy Kenneth R. Spring National Institutes of Health, Bethesda, Maryland, U.S.A. INTRODUCTION When organic or inorganic specimens absorb and sub-

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