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Chroma TECHNOLOGY CORPHANDBOOK ofOPTICAL FILTERSfor FLUORESCENCEMICROSCOPYby JAY REICHMAN 2017AN introduction TOFLUORESCENCE MICROSCOPY 2 Excitation and emission spectraBrightness of the FLUORESCENCE signalThe FLUORESCENCE microscopeTypes of filters used in FLUORESCENCE microscopyThe evolution of the FLUORESCENCE microscopeA GENERAL DISCUSSION OF OPTICAL FILTERS 8 TerminologyAvailable products Colored filter glass Thin-film coatings Acousto-optical filters Liquid Crystal Tunable FiltersDESIGNING FILTERS FORFLUORESCENCE MICROSCOPY 14 Image Contrast FLUORESCENCE spectra Light sources Detectors BeamsplittersOptical quality Optical quality parameters Optical quality requirements for wide-field microscopyFILTER SETS FOR SUB-PIXEL REGISTRATION 24 FILTERS FOR CONFOCAL MICROSCOPY 25 Optical quality requirements Nipkow-disk scanning Laser scanningSpectral requirements Nipkow-disk scanning Laser scanningFILTERS FOR MULTIPLE PROBE APPLICATIONS 29 REFERENCES 30 GLOSSARY 31 Chroma TECHNOLOGY CORPHANDBOOK ofOPTICAL FILTERSfor FLUORESCENCEMI

for FLUORESCENCE MICROSCOPY ... AN INTRODUCTION TO FLUORESCENCE MICROSCOPY 2 Excitation and emission spectra Brightness of the fluorescence signal The fluorescence microscope Types of filters used in fluorescence microscopy ... Fluorescence is a molecular phenomenon in which a substance absorbs light

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