Transcription of Gelatin Sepharose - Pennsylvania State University
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Gelatin Sepharose - Pennsylvania State University
Instructionsi 71-7094-00 Edition ADGelatin SepharoseGelatin Sepharose 4B is gelatincoupled to Sepharose 4B by thecyanogen bromide binds specifically tofibronectin, which is a highmolecular weight glycoproteinfound on the surfaces of many celltypes and present in manyextracellular fluids includingplasma. Gelatin Sepharose 4B hasbeen designed for the purification orremoval of fibronectin. p2 Table 1. Medium 8 mg Gelatin /ml drained mediumAvailable capacity:approx. 1 mg human plasma fibronectin/mldrained mediumBead structure:4% agaroseBead size range:45 165 mAverage bead size:90 mMax linear flow rate*: 75 cm/h at 25 C, HR 16/10 column,5 cm bed heightpH stability**:Long term:3 10 Short term:3 10 Chemical stability:Stable to all commonly used aqueous buffersPhysical stability:Negligible volume variation due to changes inpH or ionic strengthStorage:20% ethanol* Linear flow rate =volumetric flow rate (cm3/h)column cross-sectional area (cm2)** The ranges given are estimates based on our knowledge andexperience.
instructions i 71-7094-00 Edition AD Gelatin Sepharose Gelatin Sepharose™ 4B is gelatin coupled to Sepharose 4B by the cyanogen bromide method. Gelatin binds …
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